Effect of Sildenafil on Neuropathic Pain and Hemodynamics in Rats
황란희, 김여옥, 유명하, 이형곤, 김웅모, 최정일,
소속 상세정보
황란희 ( Huang Lan-Ji )
전남대학교 의과대학 마취통증의학교실
김여옥 ( Kim Yeo-Ok )
전남대학교 의과대학 마취통증의학교실
유명하 ( Yoon Myung-Ha )
전남대학교 의과대학 마취통증의학교실
이형곤 ( Lee Hyung-Gon )
전남대학교 의과대학 마취통증의학교실
김웅모 ( Kim Woong-Mo )
전남대학교 의과대학 마취통증의학교실
최정일 ( Choi Jeong-Il )
전남대학교 의과대학 마취통증의학교실
KMID : 0311120100510010082
Abstract
Purpose: The inhibition of phosphodiesterase 5 produces an antinociception through the increase of cyclic guanosine monophosphate (cGMP), and increasing cGMP levels enhance the release of γ-aminobutyric acid (GABA). Furthermore, this phosphodiesterase 5 plays a pivotal role in the regulation of the vasodilatation associated to cGMP. In this work, we examined the contribution of GABA receptors to the effect of sildenafil, a phosphodiesterase 5 inhibitor, in a neuropathic pain rat, and assessed the hemodynamic effect of sildenafil in normal rats.
Materials and Methods : Neuropathic pain was induced by ligation of L5/6 spinal nerves in Sprague-Dawley male rats. After observing the effect of intravenous sildenafil on neuropathic pain, GABAA receptor antagonist (bicuculline) and GABAB receptor antagonist (saclofen) were administered prior to delivery of sildenafil to determine the role of GABA receptors in the activity of sildenafil. For hemodynamic measurements, catheters were inserted into the tail artery. Mean arterial pressure (MAP) and heart rate (HR) were measured over 60 min following administration of sildenafil.
Results: Intravenous sildenafil dose-dependently increased the withdrawal threshold to the von Frey filament application in the ligated paw. Intravenous bicuculline and saclofen reversed the antinociception of sildenafil. Intravenous sildenafil increased the magnitude of MAP reduction at the maximal dosage, but it did not affect HR response.
Conclusion: These results suggest that sildenafil is active in causing neuropathic pain. Both GABAA and GABAB receptors are involved in the antinociceptive effect of sildenafil. Additionally, intravenous sildenafil reduces MAP without affecting HR.
키워드
Antinociception;GABA receptor;hemodynamics;neuropathic pain;sildenafil;Sprague-Dawley rat
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