QTLs for hybrid fertility and their association with female and male sterility in rice
Reflinur, 진중현, 장선미, 김백기, 이주현, 고희종,
소속 상세정보
( Reflinur )
Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development
진중현 ( Chin Joong-Hyoun )
International Rice Research Institute Genetics and Biotechnology Plant Breeding
장선미 ( Jang Sun-Mi )
Seoul National University Department of Plant Science
김백기 ( Kim Back-Ki )
Seoul National University Department of Plant Science
이주현 ( Lee Joo-Hyun )
Konkuk University Department of Applied Bioscience
고희종 ( Koh Hee-Jong )
Seoul National University Department of Plant Science
Abstract
Hybrid sterility is one of the major barriers to the application of wide crosses in plant breeding and is commonly encountered in crosses between indica and japonica rice varieties. Ten mapping populations comprised of two reciprocal F2 and eight BC1F1 populations generated from the cross between Ilpumbyeo (japonica) and Dasanbyeo (indica) were used to identify QTLs and to interpret the gametophytic factors involved in hybrid fertility or sterility between two subspecies. Frame maps were constructed using a total of 107 and 144 STS markers covering 12 rice chromosomes in two reciprocal F2 and eight BC1F1 populations, respectively. A total of 15 main-effect QTLs and 17 significant digenic-epistatic interactions controlling spikelet fertility (SF) were resolved in the entire genome map of F2 and BC1F1 populations. Among detected QTLs responsible for hybrid fertility, four QTLs, qSF5.1 and qSF5.2 on chromosome 5, qSF6.2 on chromosome 6, and qSF12.2 on chromosome 12, were identified as major QTLs since they were located at corresponding positions in at least three mapping populations. Loci qSF5.1, qSF6.1 and qSF6.2 were responsible for both female and male sterility, whereas qSF3.1, qSF7 and qSF12.2 affected the spikelet fertility only through embryosac factors, and qSF9.1 did through pollen factors. Five new QTLs identified in this study will be helpful for understanding the hybrid sterility and for breeding programs via inter-subspecific hybridization.
키워드
Rice; Hybrid sterility; QTL; Digenic-epistatic interaction; Gametophytic factors
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