Unmethylated state of 5′ upstream CpG islands may be necessary but not sufficient for the testis-enriched expression of ZNF230/Znf230
Liu Yunqiang, Tao Dachang, Yang Yuan, Ma Yongxin, Zhang Sizhong,
소속 상세정보
( Liu Yunqiang )
Sichuan University West China Medical School West China Hospital State Key Laboratory of Biotherapy
( Tao Dachang )
Sichuan University West China Medical School West China Hospital State Key Laboratory of Biotherapy
( Yang Yuan )
Sichuan University West China Medical School West China Hospital State Key Laboratory of Biotherapy
( Ma Yongxin )
Sichuan University West China Medical School West China Hospital State Key Laboratory of Biotherapy
( Zhang Sizhong )
Sichuan University West China Medical School West China Hospital State Key Laboratory of Biotherapy
Abstract
The testis-enriched genes ZNF230/Znf230 are located on human chromosome 11p15/mouse chromosome 7 near conserved imprinting control regions. Typical CpG islands (CGIs) extend from the promoter to the first exon in each of these genes. To investigate the correlation between the methylation status of the above CGIs and the expression patterns of the two genes, we performed bisulfite genomic sequencing of genomic DNA from human and mouse tissues and cells. The results showed that the CGIs of ZNF230/Znf230 were completely unmethylated in all selected tissues and cells, regardless of the expression levels of the two genes. Further experiments using Znf230-second-exon-knockout mice to investigate the imprinting status of Znf230 showed that its expression was not affected by genomic imprinting. However, an in vitro methylation assay illustrated that the methylation of these CpG sites could repress the expression of the luciferase reporter gene. Furthermore, chromatin immunoprecipitation with anti-Specificity protein 1 (Sp1) antibody showed that Sp1 could bind to the CGIs in the ZNF230/Znf230 gene promoter. Thus, we propose that the unmethylated state of ZNF230/Znf230 CGIs may be a prerequisite for their expression but not sufficient for their abundant expression in the testis, and that Sp1 binding may be one factor involved in preserving the methylation-free state of ZNF230/Znf230 CGIs.
키워드
Testis-enriched gene; ZNF230/Znf230; DNA methylation; Genomic imprinting; Sp1
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