Reduced fertility caused by meiotic defects and micronuclei formation during microsporogenesis in xBrassicoraphanus
신호섭, 박혜랑, 박정은, 유승화, 이기범, 김정효, 고원준, 김현희, 이수성, 허진회,
소속 상세정보
신호섭 ( Shin Ho-Sub )
Seoul National University Department of Agriculture, Forestry and Bioresources
박혜랑 ( Park Hye-Rang )
Seoul National University Department of Agriculture, Forestry and Bioresources
박정은 ( Park Jeong-Eun )
Seoul National University Department of Agriculture, Forestry and Bioresources
유승화 ( Yu Seung-Hwa )
Seoul National University Interdisciplinary Program in Agricultural Genomics
이기범 ( Yi Gi-Bum )
Seoul National University Plant Genomics and Breeding Institutes
김정효 ( Kim Jung-Hyo )
Seoul National University Interdisciplinary Program in Agricultural Genomics
고원준 ( Koh Won-Jun )
Seoul National University Department of Agriculture, Forestry and Bioresources
김현희 ( Kim Hyun-Hee )
Sahmyook University Department of Life Science
이수성 ( Lee Soo-Seong )
BioBreeding Institute
허진회 ( Huh Jin-Hoe )
Seoul National University Department of Agriculture, Forestry and Bioresources
Abstract
Background: Hybridization and polyploidization events are important driving forces in plant evolution. Allopolyploids formed between different species can be naturally or artificially created but often suffer from genetic instability and infertility in successive generations. xBrassicoraphanus is an intergeneric allopolyploid obtained from a cross between Brassica rapa and Raphanus sativus, providing a useful resource for genetic and genomic study in hybrid species.
Objective: The current study aims to understand the cause of hybrid sterility and pollen abnormality in different lines of synthetic xBrassicoraphanus from the cytogenetic perspective.
Methods: Alexander staining was used to assess the pollen viability. Cytogenetic analysis was employed to monitor meiotic chromosome behaviors in pollen mother cells (PMCs). Origins of parental chromosomes in xBrassicoraphanus meiocytes were determined by genome in situ hybridization analysis.
Results: The xBrassicoraphanus lines BB#4 and BB#6 showed high rates of seed abortion and pollen deformation. Abnormal chromosome behaviors were observed in their PMCs, frequently forming univalents and inter-chromosomal bridges during meiosis. A positive correlation also exists between meiotic defects and the formation of micronuclei, which is conceivably responsible for unbalanced gamete production and pollen sterility.
Conclusion: These results suggest that unequal segregation of meiotic chromosomes, due in part to non-homologous interactions, is responsible for micronuclei and unbalanced gamete formation, eventually leading to pollen degeneration and inferior fertility in unstable xBrassicoraphanus lines.
키워드
Intergeneric hybrids; Pollen fertility; Meiosis; Micronucleus; xBrassicoraphanus
원문 및 링크아웃 정보
등재저널 정보