Chitosan Oligosaccharides Inhibit Adipogenesis in 3T3-L1 Adipocytes
조은재, Atiar Rahman, 김상우, 백유미, 황혜진, 오정영, 황희선, 이성학, 윤종원,
소속 상세정보
조은재 ( Cho Eun-Jae )
대구대학교 생명공학과
( Atiar Rahman )
대구대학교 생명공학과
김상우 ( Kim Sang-Woo )
대구대학교 생명공학과
백유미 ( Baek Yu-Mi )
대구대학교 생명공학과
황혜진 ( Hwang Hye-Jin )
대구대학교 생명공학과
오정영 ( Oh Jung-Young )
대구대학교 생명공학과
황희선 ( Hwang Hee-Sun )
대구대학교 생명공학과
이성학 ( Lee Sung-Hak )
대구대학교 생명공학과
윤종원 ( Yun Jong-Won )
대구대학교 생명공학과
KMID : 0545120080180010080
Abstract
The 3T3-L1 cell line is a well-established and commonly used in vitro model to assess adipocyte differentiation. Over the course of several days, confluent 3T3-L1 cells can be converted to adipocytes in the presence of an adipogenic cocktail. In this study, the effects of chitosan oligosaccharides (CO) on adipocyte differentiation of 3T3-L1 cells were studied. The CO significantly decreased lipid accumulation, a marker of adipogenesis, in a dose-dependent manner. The low molecular mass CO (1-3 kDa) were the most effective at inhibiting adipocyte differentiation. Moreover, mRNA expression levels of both CCAAT/enhancer-binding protein (C/EBP) α and peroxisome proliferator-activated receptor (PPAR) γ, the key adipogenic transcription factors, were markedly decreased by CO treatments. CO also significantly downregulated adipogenic marker proteins such as leptin, adiponectin, and resistin. Our results suggest a role for CO as anti-obesity agents by inhibiting adipocyte differentiation mediated through the downregulated expression of adipogenic transcription factors and other specific genes.
키워드
Adipocyte;chitosan oligosaccharide;obesity;3T3-L1 cell;PPARγ;C/EBPα
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