Comparative Proteome Analysis of Two Antagonist Bacillus subtilis Strains
Zhang C.X., Zhao X., Han F., Yang M.F., Chen H., Chida T., Shen S.H.,
소속 상세정보
( Zhang C.X. )
Chinese Academy of Sciences Institute of Botany
( Zhao X. )
Chinese Academy of Sciences Institute of Botany
( Han F. )
Chinese Academy of Sciences Institute of Botany
( Yang M.F. )
Chinese Academy of Sciences Institute of Botany
( Chen H. )
Chinese Academy of Sciences Institute of Botany
( Chida T. )
Japan Chemicals Agrochemicals Planning and Development Department
( Shen S.H. )
Chinese Academy of Sciences Institute of Botany
KMID : 0545120090190040351
Abstract
Natural wild-type strains of Bacillus subtilis are extensively used in agriculture as biocontrol agents for plants. This study examined two antagonist B. subtilis strains, KB-1111 and KB-1122, and the results illustrated that KB-1122 was a more potent inhibitor of the indicator pathogen than KB- 1111. Thus, to investigate the intrinsic differences between the two antagonist strains under normal culture conditions, samples of KB-1111 and KB-1122 were analyzed using MALDI-TOF-MS. The main differences were related to 20 abundant intracellular and 17 extracellular proteins. When searching the NCBI database, a number of the differentially expressed proteins were identified, including 11 cellular proteins and 10 secretory proteins. Among these proteins, class III stress-response-related ATPase, aconitate hydratase, alpha-amylase precursor, and a secretory protein, endo-1, 4-beta-glucanase, were differentially expressed by the two strains. These results are useful to comprehend the intrinsic diferrences between the antagonism of KB-1111 and KB-1122.
키워드
Bacillus subtilis;Magnaporthe grisea P131;in vitro antifungal activity;proteomics;2DE-PAGE
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