Production of Biosurfactant Lipopeptides Iturin A, Fengycin and Surfactin A from Bacillus subtilis CMB32 for Control of Colletotrichum gloeosporioides
김평일, Ryu Jae-Won, 지연태, 김영환,
소속 상세정보
김평일 ( Kim Pyoung-Il )
서울대학교 생명과학부 유전공학연구소
( Ryu Jae-Won )
전남대학교 생명과학생명기술학부
지연태 ( Chi Youn-Tae )
전남대학교 생명과학생명기술학부
김영환 ( Kim Young-Hwan )
한국기초과학지원연구원
KMID : 0545120100200010138
Abstract
A bacterial strain isolated from soil for its potential to control the anthracnose disease caused by Colletotrichum gloeosporioides was identified as a Bacillus subtilis. Bacillus subtilis CMB32 produced antifungal agents on M9 broth at 30˚C. Biosurfactant lipopeptides produced by Bacillus subtilis CMB32 were precipitated by adjusting to pH 2 and extracting using chloroform/methanol, and then were purified using column chromatography and reverse-phase HPLC. Molecular masses of the lipopeptides were estimated by MALDI-TOF mass spectrometry as (a) 1080, (b) 1486, and (c) 1044 Da, respectively. They had cyclic structures and amino acid compositions of (a) Pro, Asx, Ser, Tyr, Glx, (b) Glx, Tyr, Thr, Ala, Pro, Ile, and (c) Glx, Leu, Val, Asx, respectively. Further analysis revealed that Bacillus subtilis CMB32 produced three antifungal lipopeptides: (a) iturin A, (b) fengycin, and (c) surfactin A.
키워드
Bacillus subtilis CMB32;Colletotrichum gloeosporioides;Iturin A;Fengycin;Surfactin A;MALDI-TOF mass spectrometry
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