Improving Protein Production on the Level of Regulation of both Expression and Secretion Pathways in Bacillus subtilis
Yafeng Song, Jonas M. Nikoloff, Dawei Zhang,
소속 상세정보
( Yafeng Song )
Chinese Academy of Sciences Tianjin Institute of Industrial Biotechnology
( Jonas M. Nikoloff )
Chinese Academy of Sciences Tianjin Institute of Industrial Biotechnology
( Dawei Zhang )
Chinese Academy of Sciences Tianjin Institute of Industrial Biotechnology
KMID : 0545120150250070963
Abstract
The well-characterized gram-positive bacterium Bacillus subtilis is an outstanding industrial candidate for protein expression owing to its single membrane and high capacity of secretion, simplifying the downstream processing of secretory proteins. During the last few years, there has been continuous progress in the illustration of secretion mechanisms and application of this robust host in various fields of life science, such as enzyme production, feed additives, and food and pharmaceutical industries. Here, we review the developments of Bacillus subtilis as a highly promising expression system illuminating strong chemical- and temperatureinducible and other types of promoters, strategies for ribosome-binding-site utilization, and the novel approach of signal peptide selection. Furthermore, we outline the main steps of the Sec pathway and the relevant elements as well as their interactions. In addition, we introduce the latest discoveries of Tat-related complex structures and functions and the countless applications of this full-folded protein secretion pathway. This review also lists some of the current understandings of ATP-binding cassette transporters. According to the extensive knowledge on the genetic modification strategies and molecular biology of Bacillus subtilis, we propose some suggestions and strategies for improving the yield of intended productions. We expect this to promote striking future developments in the optimization and application of this bacterium.
키워드
promoter; ribosome-binding site; signal peptide; Sec pathway; Tat pathway; ATP-binding cassette transporter
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