Increased Immunogenicity and Protective Efficacy of a P. aeruginosa Vaccine in Mice Using an Alum and De-O-Acylated Lipooligosaccharide Adjuvant System
류지인, Wui Seo-Ri, 고아라, Do Hien Thi Thu, 이연정, Kim Hark-Jun, Rhee In-Moo, 박신애, 김광성, 조양제, 이나경,
소속 상세정보
류지인 ( Ryu Ji-In )
Sejong University Department of Bioscience and Biotechnology
( Wui Seo-Ri )
Sejong University Department of Bioscience and Biotechnology
고아라 ( Ko A-Ra )
Sejong University Department of Bioscience and Biotechnology
( Do Hien Thi Thu )
Sejong University Department of Bioscience and Biotechnology
이연정 ( Lee Yeon-Jeong )
Sejong University Department of Bioscience and Biotechnology
( Kim Hark-Jun )
Sejong University Department of Bioscience and Biotechnology
( Rhee In-Moo )
Sejong University Department of Bioscience and Biotechnology
박신애 ( Park Shin-Ae )
Eyegene Inc.
김광성 ( Kim Kwang-Sung )
Eyegene Inc.
조양제 ( Cho Yang-Je )
Eyegene Inc.
이나경 ( Lee Na-Gyong )
Sejong University Department of Bioscience and Biotechnology
Abstract
Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen that commonly causes fatal infections in cystic fibrosis and burn patients as well as in patients who are hospitalized or have impaired immune systems. P. aeruginosa infections are difficult to treat owing to the high resistance of the pathogen to conventional antibiotics. Despite several efforts, no effective prophylactic vaccines against P. aeruginosa are currently available. In this study, we investigated the activity of the CIA06 adjuvant system, which is composed of alum and de-Oacylated lipooligosaccharide, on a P. aeruginosa outer membrane protein (OMP) antigen vaccine in mice. The results indicated that CIA06 significantly increased the antigen-specific IgG titers and opsonophagocytic activity of immune sera against P. aeruginosa. In addition, the antibodies induced by the CIA06-adjuvanted vaccine exhibited higher cross-reactivity with heterologous P. aeruginosa strains. Finally, mice immunized with the CIA06-adjuvanted vaccine were effectively protected from lethal P. aeruginosa challenge. Based on these data, we suggest that the CIA06 adjuvant system might be used to promote the immunogenicity and protective efficacy of the P. aeruginosa OMP vaccine.
키워드
Pseudomonas aeruginosa; vaccine; adjuvant; de-O-acylated lipooligosaccharide; protective efficacy
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