Experimental Applications of in situ Liver Perfusion Machinery for the Study of Liver Disease
최원묵, 은혁수, 이영선, 김선준, 김명호, 이준희, 심영리, 김희훈, 김예은, 이현승, 정원일,
소속 상세정보
최원묵 ( Choi Won-Mook )
Korea Advanced Institute of Science and Technology Graduate School of Medical Science and Engineering Laboratory of Liver Research
은혁수 ( Eun Hyuk-Soo )
Chungnam National University College of Medicine Department of Internal Medicine
이영선 ( Lee Young-Sun )
Korea University College of Medicine Department of Internal Medicine
김선준 ( Kim Sun-Jun )
Chungnam National University College of Medicine Department of Internal Medicine
김명호 ( Kim Myung-Ho )
Korea Advanced Institute of Science and Technology Graduate School of Medical Science and Engineering Laboratory of Liver Research
이준희 ( Lee Jun-Hee )
Korea Advanced Institute of Science and Technology Graduate School of Medical Science and Engineering Laboratory of Liver Research
심영리 ( Shim Young-Ri )
Korea Advanced Institute of Science and Technology Graduate School of Medical Science and Engineering Laboratory of Liver Research
김희훈 ( Kim Hee-Hoon )
Korea Advanced Institute of Science and Technology Graduate School of Medical Science and Engineering Laboratory of Liver Research
김예은 ( Kim Ye-Eun )
Korea Advanced Institute of Science and Technology Graduate School of Medical Science and Engineering Laboratory of Liver Research
이현승 ( Yi Hyon-Seung )
Chungnam National University College of Medicine Department of Internal Medicine
정원일 ( Jeong Won-Il )
Korea Advanced Institute of Science and Technology Graduate School of Medical Science and Engineering Laboratory of Liver Research
Abstract
The liver is involved in a wide range of activities in vertebrates and some other animals, including metabolism, protein synthesis, detoxification, and the immune system. Until now, various methods have been devised to study liver diseases; however, each method has its own limitations. In situ liver perfusion machinery, originally developed in rats, has been successfully adapted to mice, enabling the study of liver diseases. Here we describe the protocol, which is a simple but widely applicable method for investigating the liver diseases. The liver is perfused in situ by cannulation of the portal vein and suprahepatic inferior vena cava (IVC), with antegrade closed circuit circulation completed by clamping the infrahepatic IVC. In situ liver perfusion can be utilized to evaluate immune cell migration and function, hemodynamics and related cellular reactions in each type of hepatic cells, and the metabolism of toxic or other compounds by changing the composition of the circulating media. In situ liver perfusion method maintains liver function and cell viability for up to 2 h. This study also describes an optional protocol using density-gradient centrifugation for the separation of different types of hepatic cells, allowing the determination of changes in each cell type. In summary, this method of in situ liver perfusion will be useful for studying liver diseases as a complement to other established methods.
키워드
hemodynamics; immune cell; in situ perfusion; liver disease; metabolism
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