Chemical Composition and Antimicrobial Efficacy of Helminthostachys zeylanica against Foodborne Bacillus cereus
Yenn Tong Woei, Ring Leong Chean, Zahan Khairul Azly, Rahman Muhammad Sharir Abdul, Tan Wen-Nee, Alaudin Bintul Jauza’ Shaik,
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( Yenn Tong Woei )
Universiti Kuala Lumpur Malaysian Institute of Chemical and Bioengineering Technology
( Ring Leong Chean )
Universiti Kuala Lumpur Malaysian Institute of Chemical and Bioengineering Technology
( Zahan Khairul Azly )
Universiti Kuala Lumpur Malaysian Institute of Chemical and Bioengineering Technology
( Rahman Muhammad Sharir Abdul )
Universiti Kuala Lumpur Malaysian Institute of Chemical and Bioengineering Technology
( Tan Wen-Nee )
Universiti Sains Malaysia School of Distance Education
( Alaudin Bintul Jauza’ Shaik )
Universiti Kuala Lumpur Malaysian Institute of Chemical and Bioengineering Technology
Abstract
Helminthostachys zeylanica is a rare plant grows in lightly shaded areas. The fern was traditionally used as antipyretic and antiphlogistic agents. This study was aimed to evaluate the antibacterial potential of H. zeylanica on foodborne Bacillus cereus. The chemical composition of its ethanolic extract was also determined. The plant samples were collected at Kampung Kebun Relong, Kedah, Malaysia. The ethanolic extract showed significant inhibitory activity on B. cereus with a sizeable clear zone detected on disc diffusion assay. On broth microdilution assay, the MIC of the extract on B. cereus was 6.25 mg/ml and the MBC was 12.5 mg/ml. The inhibitory activity of the extract on B. cereus was bactericidal. In the growth dynamic study, the antibacterial efficacy of the extract was concentration dependent, where a lower colony forming unit count was obtained with increased extract concentration. The SEM micrograph of extract treated B. cereus cells showed invaginations of cell wall. The bacterial cell structure collapsed after 24 h exposure to the extract. The GCMS analysis of the extract showed that the major constituents of the extract were phenol (36.26%) and quercetin (29.70%). This study is important as it shows the potential use of H. zeylanica as an effective agent to control B. cereus related infections.
키워드
Antimicrobial activity; Bacillus cereus; Helminthostachys zeylanica
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