Overexpression of Telomerase Extends Longevity, Alters Differentiation and Gene Expression Profile in Human Adipose Tissue Stromal Cells
전은숙, 조현화, 이태훈, 정진섭,
소속 상세정보
전은숙 ( Jun Eun-Sook )
부산대학교 의과대학 생리학교실
조현화 ( Cho Hyun-Hwa )
부산대학교 의과대학 생리학교실
이태훈 ( Lee Tae-Hoon )
부산대학교 의과대학 생리학교실
정진섭 ( Jung Jin-Sup )
부산대학교 의과대학 생리학교실
KMID : 0811720030070000130
Abstract
Human bone marrow stromal cells (hBMSCs) are defined as pluripotent progenitor cells with the ability to differentiate into osteoblasts, chondrochytes, adipocytes, muscle cells, and neural cells. Recently, it has been shown that telomerase expression not only extends the replicative life-span and maintains their bone-forming capability of hBMSCs. We previeously reported that human adipose tissue stromal cells (hATSCs) have similar characteristics with hBMSCs. In this study, hATSCs were stably tranduced by a retroviral vector containing the gene for the catalytic subunit of human telomerase (hTERT). The tranduced clones (hATSC-TERTs) had high telomerase activity, which was maintained during subsequent subcultivation. The transduced cells of two representative clones have undergone more than 100 population doublings (PD) and continue to proliferate, whereas control cells underwent senescence-associated proliferation arrest after 36-40 PD. The cells had a normal karyotype, and increased differentiation potential, especially osteogenic lineage. Intraventricular injection of hATSC-TERTs in ischemic rat brain showed enhancement of functional recovery as like hATSC-MSCVs. Microarray revealed diverse changes in gene expression profile including genes related to osteogenic differentiaton. These results further support a similarity between hBMSCs and hATSCs.
키워드
Adipose tissue stromal cells;Telomerase;Differentiation
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