Comparative assessment of the intracellular survival of the Burkholderia pseudomallei bopC mutant
Varintip Srinon, Sunsiree Muangman, Nithima Imyaem, Veerachat Muangsombut, Sunee Korbsrisate, Natalie R. Lazar Adler, Edouard E. Galyov,
소속 상세정보
( Varintip Srinon )
Mahidol University Faculty of Medicine Siriraj Hospital Department of Immunology
( Sunsiree Muangman )
Mahidol University Faculty of Medicine Siriraj Hospital Department of Immunology
( Nithima Imyaem )
Mahidol University Faculty of Medicine Siriraj Hospital Department of Immunology
( Veerachat Muangsombut )
Mahidol University Faculty of Medicine Siriraj Hospital Department of Immunology
( Sunee Korbsrisate )
Mahidol University Faculty of Medicine Siriraj Hospital Department of Immunology
( Natalie R. Lazar Adler )
University of Leicester Department of Infection, Immunity and Inflammation
( Edouard E. Galyov )
University of Leicester Department of Infection, Immunity and Inflammation
KMID : 0880220130510040522
Abstract
Burkholderia pseudomallei, the causative agent of melioidosis, is a Gram-negative saprophytic bacterium capable of surviving within phagocytic cells. To assess the role of BopC (a type III secreted effector protein) in the pathogenesis of B. pseudomallei, a B. pseudomallei bopC mutant was used to infect J774A.1 macrophage-like cells. The bopC mutant showed significantly reduced intracellular survival in infected macrophages compared to wild-type B. pseudomallei. In addition, the bopC mutant displayed delayed escape from endocytic vesicles compared with the wild-type strain. This indicates that BopC is important, and at least in part, needed for intracellular survival of B. pseudomallei.
키워드
Burkholderia pseudomallei; BopC; type III secretion effector; intracellular survival; phagocyte
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