Pathogenomics of Streptococcus ilei sp. nov., a newly identified pathogen ubiquitous in human microbiome
현동욱, 이재윤, 김민수, 신나리, 원태웅, 김경현, 김필수, 탁은정, 정미자, 이준영, 김현식, 강우림, 성호준, 전체옥, 배진우,
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현동욱 ( Hyun Dong-Wook )
Kyung Hee University Department of Biology
이재윤 ( Lee Jae-Yun )
Kyung Hee University Department of Biology
김민수 ( Kim Min-Soo )
Kyung Hee University Department of Biology
신나리 ( Shin Na-Ri )
Kyung Hee University Department of Biology
원태웅 ( Whon Tae-Woong )
Kyung Hee University Department of Biology
김경현 ( Kim Kyung-Hyun )
Chung-Ang University Department of Life Science
김필수 ( Kim Pil-Soo )
Kyung Hee University Department of Biology
탁은정 ( Tak Euon-Jung )
Kyung Hee University Department of Biology
정미자 ( Jung Mi-Ja )
Kyung Hee University Department of Biology
이준영 ( Lee June-Young )
Kyung Hee University Department of Biology
김현식 ( Kim Hyun-Sik )
Kyung Hee University Department of Biology
강우림 ( Kang Woo-Rim )
Kyung Hee University Department of Biology
성호준 ( Sung Ho-Jun )
Kyung Hee University Department of Biology
전체옥 ( Jeon Che-Ok )
Chung-Ang University Department of Life Science
배진우 ( Bae Jin-Woo )
Kyung Hee University Department of Biology
Abstract
Viridans group streptococci are a serious health concern because most of these bacteria cause life-threatening infections, especially in immunocompromised and hospitalized individuals. We focused on two alpha-hemolytic Streptococcus strains (I-G2 and I-P16) newly isolated from an ileostomy effluent of a colorectal cancer patient. We examined their pathogenic potential by investigating their prevalence in human and assessing their pathogenicity in a mouse model. We also predicted their virulence factors and pathogenic features by using comparative genomic analysis and in vitro tests. Using polyphasic and systematic approaches, we identified the isolates as belonging to a novel Streptococcus species and designated it as Streptococcus ilei. Metagenomic survey based on taxonomic assignment of datasets from the Human Microbiome Project revealed that S. ilei is present in most human population and at various body sites but is especially abundant in the oral cavity. Intraperitoneal injection of S. ilei was lethal to otherwise healthy C57BL/6J mice. Pathogenomics and in vitro assays revealed that S. ilei possesses a unique set of virulence factors. In agreement with the in vivo and in vitro data, which indicated that S. ilei strain I-G2 is more pathogenic than strain I-P16, only the former displayed the streptococcal group A antigen. We here newly identified S. ilei sp. nov., and described its prevalence in human, virulence factors, and pathogenicity. This will help to prevent S. ilei strain misidentification in the future, and improve the understanding and management of streptococcal infections.
키워드
Streptococcus; Streptococcus ilei; ileum; ileostomy effluent; virulence factor; pathogenicity; viridans group streptococci; mitis group streptococci
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