Hairy Root Cultures of Taxus cuspidatafor Enhanced Production of Paclitaxel
김정아, 백광현, Son Young-Mi, 손성호, Shin Heung-Sop,
소속 상세정보
김정아 ( Kim Jung-A )
VitroSys Inc.
백광현 ( Baek Kwang-Hyun )
영남대학교 생명공학부
( Son Young-Mi )
VitroSys Inc.
손성호 ( Son Sung-Ho )
VitroSys Inc.
( Shin Heung-Sop )
한국산업기술대학교 생명화학공학과
KMID : 0903520090520020144
Abstract
Hairy root cultures were established by transforming seedlings of Taxus cuspidata Sieb. Et Zucc (Korean yew) using Agrobacterium rhizogenes.Initially, 107 lines of hairy roots were induced by infection with 3 strains of Agrobacterium (R1000, A4, and 15384), however, only three lines generated from the R1000 strain were stably grown on hormone-free media over 10 months of successive cultures. These lines were designated as RC11104, RC11105 and RC11106. The hairy root line RC 11106 was selected for further experiments for taxol production based on its growth properties. Upon methyl jasmonate treatment, the RC11106 line accumulated 52.5 mg/L of taxol over 2 weeks of incubation at a 20-L culture scale.
키워드
Agrobacterium rhizogenes;bioreactor;hairy root;methyl jasmonate;taxol;Taxus cuspidata Sieb;et Zucc
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