Production and Application of Galacto-oligosaccharides from Lactose by a Recombinant β-Galactosidase of Bifidobacterium infantis Overproduced by Pichia pastoris
정성제, Lee Byong-Hoon,
소속 상세정보
정성제 ( Jung Sung-Je )
경희대학교 식품생명공학과
( Lee Byong-Hoon )
CANADA McGill University Department of Food Science
KMID : 1007520080170030514
Abstract
After overproduction of a recombinant β-galactosidase of Bifidobacterium infantis in Pichia pastoris, a synthesis of galacto-oligosaccharides (GOS) from 36% lactose using the enzyme (170.74 U/mg) was investigated. The transgalactosylation ratio reached up to 25.2% with 83.1% conversion of initial lactose and the maximum yield of GOS was 40.6%. The GOS syrup was composed of a 13.43% galacto-oligosaccharides, 5.06% lactose, and 8.76% monosaccharides. The prebiotic effect of GOS on the growth of bifidobacteria and lactobacilli strains was investigated in vitro. The maximum growth rate of Bifidobacterium breve and Lactobacillus acidophillus in GOS syrup (5%, v/v) media were 0.49 and 0.96/hr that are higher than those in 1%(w/v) galactose and 1%(w/v) lactose containing media. However, there was no significant difference between the specific growth rates of L. acidophillus in 1%(w/v) glucose and 5%(v/v) GOS syrup. Our data showed that GOS definitely promoted the growth of B. breve ATCC 15700T and L. acidophilus ATCC 33323.
키워드
galacto-oligosaccharide;Pichia pastoris;recombinant β-galactosidase;non-digestible oligosaccharide;Bifidobacterium;Lactobacillus
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