Isolation and Characterization of Chondroitin Sulfates from the By-products of Marine Organisms
임아랑, 심준수, 한범수, 박유미, Toida Toshihiko, 김영식,
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임아랑 ( Im A-Rang )
서울대학교 약학대학 천연물과학연구소
심준수 ( Sim Joon-Soo )
서울대학교 약학대학 천연물과학연구소
한범수 ( Hahn Bum-Soo )
서울대학교 천연물과학연구소
박유미 ( Park You-Mie )
서울대학교 약학대학 천연물과학연구소
( Toida Toshihiko )
Chiba University Graduate School of Pharmaceutical Sciences
김영식 ( Kim Yeong-Shik )
서울대학교 약학대학 천연물과학연구소
KMID : 1007520090180040872
Abstract
By-products of marine organisms including salmon, skate, flatfish, and yellow goosefish were investigated tosearch for new source of chondroitin sulfate (CS). Agarose gel electrophoresis with chondroitinase depolymerization showedthat purified chondroitin sulfate did not contain any other glycosaminoglycans. 1H-nuclear magnetic resonance (NMR) spectrawere acquired to confirm the structure and purity. The average molecular weight ranging from 22 to 64 kDa was determinedby high performance size exclusion chromatography. Disaccharide compositions and purities were determined by strong anionexchange-high performance liquid chromatography (SAX-HPLC) after chondroitinase ABC depolymerization. SAX-HPLCdata exhibited that the purity was from 81.7±1.3 to 114.2±2.5% and the yield was from 1.3 to 12.5%. All analytical resultsindicate that salmon cartilage, skate cartilage, and yellow goosefish bone could be promising sources of CS to substitute sharkcartilage CS in commercial neutraceuticals.
키워드
chondroitin sulfate;raw material;marine organism;characterization
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