어성초 메탄올 추출물에 의한 A549 인체 폐암세포 사멸유도에 관한 연구
Induction of Apoptotic Cell Death by Methanol Extract of Houttuynia cordata Thunb. in A549 Human Lung Carcinoma Cells
홍수현, 박철, 홍상훈, 최병태, 이용태, 박동일, 최영현,
소속 상세정보
홍수현 ( Hong Su-Hyun )
동의대학교 한의과대학 생화학교실
박철 ( Park Cheol )
동의대학교 한의과대학 생화학교실
홍상훈 ( Hong Sang-Hoon )
동의대학교 한의과대학 내과학교실
최병태 ( Choi Byung-Tae )
동의대학교 한의과대학 해부학교실
이용태 ( Lee Yong-Tae )
동의대학교 한의과대학 생리학교실
박동일 ( Park Dong-Il )
동의대학교 한의과대학 내과학교실
최영현 ( Choi Yung-Hyun )
동의대학교 한의과대학 생화학교실
KMID : 1123920060200061584
Abstract
Houttuynia cordata Thunb, well known as ’E-Sung-Cho’ in Korea, is traditional medicinal plant generally used in Oriental medicine therapy. We previously reported that the water extract of H. cordata inhibited cell proliferation and induced apoptosis in human breast carcinoma cells. In the present study, we investigated the biochemical mechanisms of anti-proliferative effects by the methanol extract of H. cordata (MEHC) in human lung carcinoma A549 cells. It was found that MEHC could inhibit the cell growth in a dose-dependent manner, which was associated with morphological change and apoptotic cell death as determined by formation of apoptotic bodies, DNA fragmentation and increased populations of apoptotic-sub G1 phase cells. Apoptosis of A549 cells by MEHC was also connected with a down-regulation of anti-apoptotic Bcl-2 and inhibitor of apoptosis proteins (IAPs) expression. MEHC treatment induced the proteolytic activation of caspase-3, caspase-8 and caspase-9, and a concomitant inhibition of poly(ADP-ribose) polymerase (PARP), -catenin and phospholipase (PLC)-1 protein expression. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of H. cordata.
키워드
Houttuynia cordata Thunb;apoptosis;A549;Bcl-2;IAPs;caspases
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