Decreased Levels of Spleen Tissue CD4+CD25+Foxp3+ Regulatory T Lymphocytes in Mice Exposed to Berberin
Karimi Gholamreza, Mahmoudi Mahmoud, Balali-Mood Mahdi, Rahnama Maryam, Rabe Shahrzad Zamani Taghizadeh Rabe, Tabasi Nafiseh, Riahi-Zanjani Bamdad,
소속 상세정보
( Karimi Gholamreza )
Mashhad University of Medical Sciences Pharmacy School Pharmaceutical Research Center
( Mahmoudi Mahmoud )
Mashhad University of Medical Sciences Immunology Research Center
( Balali-Mood Mahdi )
Mashhad University of Medical Sciences School of Medicine Medical Toxicology Research Center
( Rahnama Maryam )
Mashhad University of Medical Sciences Bu-Ali Research Institute Immunology Research Center
( Rabe Shahrzad Zamani Taghizadeh Rabe )
Mashhad University of Medical Sciences Bu-Ali Research Institute Immunology Research Center
( Tabasi Nafiseh )
Mashhad University of Medical Sciences Bu-Ali Research Institute Immunology Research Center
( Riahi-Zanjani Bamdad )
Mashhad University of Medical Sciences School of Medicine Medical Toxicology Research Center
Abstract
The effects of isoquinoline alkaloid berberine (BER) on spleen tissue CD4+CD25+Foxp3+ regulatory T (Treg) cells were evaluated in BALB/c mice. Here, BER was administered daily by intraperitoneal injection at doses of 5 mg/kg and 10 mg/kg for 14 days. Following the exposure, mice spleen cellularities, IL-10 production by splenocytes, and spleen Treg/CD4+ cell profiles were studied in all the test groups of animals. The results showed that a high dose of BER (10 mg/kg) could decrease both the absolute and relative percentages of spleen Treg cells as well as decrease the production of IL-10 by splenocytes in the treated mice (p<0.05). BER at 5 mg/kg did not appear to affect any of these parameters. Based on the finding here, it would seem that BER has effective immunostimulatory properties, which contradicts the results from other studies indicating immunosuppressive effects of BER. Depending on the doses of BER used, it might have a broad spectrum from immunosuppressive to stimulatory effects. Further studies, including more doses, are required to better evaluate the effects of this natural product. Mechanistic studies are required, particularly in case of redox state of the immune cells, to elucidate and determine how BER functions to impart the toxicity effects demonstrated here and in other studies.
키워드
berberine; BALB/c mice; spleen; Treg cells
원문 및 링크아웃 정보
등재저널 정보