Evaluation of Antioxidant, Anti-cholinesterase, and Anti-inflammatory Effects of Culinary Mushroom Pleurotus pulmonarius
Nguyen Trung Kien, Im Kyung-Hoan, 최재혁, 신평균, 이태수,
소속 상세정보
( Nguyen Trung Kien )
Incheon National University Division of Life Sciences
( Im Kyung-Hoan )
Incheon National University Division of Life Sciences
최재혁 ( Choi Jae-Hyuk )
Incheon National University Division of Life Sciences
신평균 ( Shin Pyung-Gyun )
Rural Development Administration National Institute of Horticultural & Herbal Science Mushroom Research Division
이태수 ( Lee Tae-Soo )
Incheon National University Division of Life Sciences
Abstract
Culinary mushroom Pleurotus pulmonarius has been popular in Asian countries. In this study, the anti-oxidant, cholinesterase, and inflammation inhibitory activities of methanol extract (ME) of fruiting bodies of P. pulmonarius were evaluted. The 1,1-diphenyl-2-picryl-hydrazy free radical scavenging activity of ME at 2.0 mg/mL was comparable to that of butylated hydroxytoluene, the standard reference. The ME exhibited significantly higher hydroxyl radical scavenging activity than butylated hydroxytoluene. ME showed slightly lower but moderate inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase than galantamine, a standard AChE inhibitor. It also exhibited protective effect against cytotoxicity to PC-12 cells induced by glutamate (10~100 μg/mL), inhibitory effect on nitric oxide (NO) production and inducible nitric oxide synthase protein expression in lipopolysaccharide-stimulated RAW 264.7 macrophages, and carrageenan-induced paw edema in a rat model. Highperformance liquid chromatography analysis revealed the ME of P. pulmonarius contained at least 10 phenolic compounds and some of them were identified by the comparison with known standard phenolics. Taken together, our results demonstrate that fruiting bodies of P. pulmonarius possess antioxidant, anti-cholinesterase, and inflammation inhibitory activities.
키워드
Anti-cholinesterase; Anti-inflammation; Antioxidant; Cytotoxicity; Pleurotus pulmonarius
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