Detection of Helicobacter pylori in Gastric Aspirates Using a Monoclonal Antibody-Based Test
김호동, 김도현, 박혜욱, 김우종, Ahn Yong-Soo, 이영직, 박선미, Seo Eun-Seon, 박철, 김양호, 김형락, 주영은, 정영도,
소속 상세정보
김호동 ( Kim Ho-Dong )
Saint Carollo Hospital Department of Internal Medicine
김도현 ( Kim Do-Hyun )
Saint Carollo Hospital Department of Internal Medicine
박혜욱 ( Park Hye-uk )
Saint Carollo Hospital Department of Internal Medicine
김우종 ( Kim Woo-Jong )
Saint Carollo Hospital Department of Internal Medicine
( Ahn Yong-Soo )
Saint Carollo Hospital Department of Internal Medicine
이영직 ( Lee Young-Jik )
Saint Carollo Hospital Department of Internal Medicine
박선미 ( Park Sun-Mi )
Saint Carollo Hospital Department of Internal Medicine
( Seo Eun-Seon )
Saint Carollo Hospital Department of Internal Medicine
박철 ( Park Chul )
Saint Carollo Hospital Department of Internal Medicine
김양호 ( Kim Yang-Ho )
Saint Carollo Hospital Department of Internal Medicine
김형락 ( Kim Hyung-Rag )
Saint Carollo Hospital Department of Internal Medicine
주영은 ( Joo Young-Eun )
Chonnam National University Medical School Department of Internal Medicine
정영도 ( Jung Young-Do )
Chonnam National University Medical School Department of Biochemistry
KMID : 1188320130070010030
Abstract
Background/Aims:The objective of this study was to evaluate a monoclonal antibody-based test to detect Helicobacter pylori-specific antigen in gastric aspirates from humans.
Methods:Sixty-one volunteers were enrolled in the study. All of the subjects underwent a 13C-urea breath test (UBT) before esophagogastroduodenoscopy. Gastric aspirates were analyzed for pH and ammonia and used for polymerase chain reaction (PCR), culture, and monoclonal antibody-based detection of H. pylori. Multiple biopsies of the gastric antrum and body were obtained for a rapid urease test (RUT) and histological evaluation.
Results:Thirty-six subjects were H. pylori-positive and 25 were H. pylori-negative according to the UBT results. Compared with the H. pylori-negative subjects, H. pylori-positive subjects had a higher pH (4.77±1.77 vs 3.49±1.30, p<0.05) and ammonia level (1,130.9±767.4 vs 184.2±126.3, p<0.0001). The sensitivities and specificities of the PCR test, RUT, culture test, and monoclonal antibody-based test were 100% and 72%, 89% and 100%, 47% and 100%, and 78% and 100%, respectively.
Conclusions:The monoclonal antibody-based test for diagnosing H. pylori infection in gastric aspirates has increased sensitivity compared with the culture test and specificity as high as that of the RUT. The test may be useful as an additive test for examining gastric aspirates.
키워드
Helicobacter pylori; Gastric aspirate; Monoclonal antibody-based test
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