The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice
Zhang Didi, 이지현, 신형은, 곽성은, 배준현, Tang Liang, 송욱,
소속 상세정보
( Zhang Didi )
Seoul National University Institute of Sport Science
이지현 ( Lee Ji-Hyun )
Seoul National University Institute of Sport Science
신형은 ( Shin Hyung-Eun )
Seoul National University Institute of Sport Science
곽성은 ( Kwak Seong-Eun )
Seoul National University Institute of Sport Science
배준현 ( Bae Jun-Hyun )
Seoul National University Institute of Sport Science
( Tang Liang )
Shaanxi Normal University Institute of Sports Biology
송욱 ( Song Wook )
Seoul National University Institute of Sport Science
Abstract
Background: Autophagy maintains muscle mass and healthy skeletal muscles. Several recent studies have associated sugar-sweetened beverage (SSB) consumption with diseases. We investigated whether muscle dysfunction due to obesity could be restored by SSB restriction (SR) alone or in combination with exercise (EX) training.
Methods: Obese mice were subjected to SR combined with treadmill EX. Intraperitoneal glucose tolerance test, grip strength test, hanging time test, and body composition analysis were performed. Triglyceride (TG) and total cholesterol (TC) serum concentrations and TG concentrations in quadriceps muscles were analyzed. Western blot and reverse transcription-quantitative polymerase chain reaction helped analyze autophagy-related protein and mRNA expression, respectively.
Results: SR alone had no significant effect on fasting blood glucose levels, glucose tolerance, and muscle function. However, it had effect on serum TC, serum TG, and BCL2 interacting protein 3 expression. SR+EX improved glucose tolerance and muscle function and increased serum TC utilization than SR alone. SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group.
Conclusion: SR induced mitophagy-related protein expression in quadriceps, without affecting muscle function. And, the combination of SR and EX activated mitophagy-related proteins and improved muscle function.
키워드
Autophagy; Diet, high-fat; Muscle, skeletal; Obesity; Sucrose; Sugar-sweetened beverages
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