인체 대장 상피세포주에서 C-X-C Chemokine 유전자의 발현 및 조절에 관한 예비 연구
A Preliminary Study on the Expression and Regulation of C-X-C Chemokine Genes in a Human Colon Epithelial Cell Line
양석균, 김옥희, 이미화, 박선미, 정훈용, 홍원선, 민영열,
소속 상세정보
양석균 ( Yang Suk-Kyun )
울산대학교 의과대학 서울중앙병원 내과학교실
김옥희 ( Kim Ok-Hee )
아산생명과학연구소
이미화 ( Lee Mi-Hwa )
울산대학교 의과대학 서울중앙병원 내과학교실
박선미 ( Park Sun-Mi )
울산대학교 의과대학 서울중앙병원 내과학교실
정훈용 ( Jung Hwoon-Yong )
울산대학교 의과대학 서울중앙병원 내과학교실
홍원선 ( Hong Weon-Seon )
울산대학교 의과대학 서울중앙병원 내과학교실
민영열 ( Min Young-Il )
울산대학교 의과대학 서울중앙병원 내과학교실
KMID : 0356719980140010021
Abstract
Human colon epithelial cells secrete an array of proinflammatory cytokines that includes IL-8, MCP-1, GM-CSF, TNFα and IL-6. This response may serve to attract neutrophils and macrophags to the site of infection. In addition to IL-8 and MCP-1, the chemokine family contains other members, which, alone or in combination, can recruit and/or activate inflammatory and lymphoid cells. In this study, we asked whether colon epithelial cells express a broader array of chemokines than previously described. The colon epithelial cell line, Caco-2, was stimulated for 3h with IL-1α, or was infected with Salmonella dublin. RNA was extracted and chemokine mRNA levels were determined by quantitative reverse transcription-PCR using internal RNA standards. Ex pression of GROα, GROβ, GROβ and IP-10 increased by bacterial infection or IL-lα stimulation. These data strongly support the notion that epithelal cells are an important and integral component of the host’s natural immune system.
키워드
Chemokine;Epithelial cell;Colon
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