궤양성 대장염 환자의 대장점막에서 Chemokine 유전자의 발현 양상
The Increased Expression of Chemokines in the Colonic Mucosa of Patients with Ulcerative Colitis
심기남, 양석균, 명승재, 김옥희, 오현주, 이정아, 조윤경, 유창식, 정훈용, 홍원선, 김진호, 민영열,
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심기남 ( Sim Ki-Nam )
울산대학교 의과대학 서울아산병원 내과학교실
양석균 ( Yang Suk-Kyun )
울산대학교 의과대학 서울아산병원 내과학교실
명승재 ( Myung Seung-Jae )
울산대학교 의과대학 서울아산병원 내과학교실
김옥희 ( Kim Ok-Hee )
아산생명과학연구소
오현주 ( Oh Hyun-Joo )
아산생명과학연구소
이정아 ( Lee Jeung-A )
아산생명과학연구소
조윤경 ( Jo Youn-Kyung )
아산생명과학연구소
유창식 ( Yu Chang-Sik )
울산대학교 의과대학 서울아산병원 외과학교실
정훈용 ( Jung Hwoon-Yong )
울산대학교 의과대학 서울아산병원 내과학교실
홍원선 ( Hong Won-Sun )
울산대학교 의과대학 서울아산병원 내과학교실
김진호 ( Kim Jin-Ho )
울산대학교 의과대학 서울아산병원 내과학교실
민영열 ( Min Young-Il )
울산대학교 의과대학 서울아산병원 내과학교실
KMID : 0356720020180030147
Abstract
Purpose: To better understand the extent to which chemokines participate in the mucosal inflammatory response in patients with ulcerative colitis (UC), we assessed the expression of an array of chemokines in the colonic mucosa of UC patients.
Methods: Colonic mucosal biopsy specimens were obtained from 15 patients with UC and 12 normal controls. Messenger RNA (mRNA) levels for 10 chemokines were quantitated by reverse-transcription PCR using synthetic standard RNAs. The biopsy specimens were also cultured, and secreted chemokines in culture supernatants were assayed by ELISA.
Results: The mRNA expression of C-X-C (IL-8, GROα, GROβ, GROγ, ENA-78, and IP-10) and C-C (MCP-1, MIP-1β, and RANTES), but not C (lymphotactin) chemokines was significantly higher in the affected mucosa of UC patients than in the unaffected mucosa of UC patients or in the normal mucosa of normal controls. The degree of increased expression was more prominent in the C-X-C than in the C-C chemokines. Further, the secretion of IL-8, GROα, ENA-78, and MCP-1 was higher in UC patients than in normal controls. Secretions of MIP-1β and RANTES also showed a trend toward an increase in UC, but it did not reach statistical significance.
Conclusions: The increased expression of a variety of chemokines in UC suggest that chemokines may play an important role in the immunopathogenesis of UC.
키워드
궤양성 대장염;대장
Chemokine;Ulcerative colitis;Colon
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