복막전이를 보이는 대장암에서 E-cadherin 단백 발현 및 CDH1 유전자의 변이 검색
Loss of E-cadherin Function is Suggested to be Associated with Peritoneal Seeding in Colorectal Cancer
김희철, 노선애, 김정선, 유창식, 김진천,
소속 상세정보
김희철 ( Kim Hee-Cheol )
울산대학교 의과대학 외과학교실
노선애 ( Roh Seon-Ae )
울산대학교 의과대학 서울아산병원 외과학교실
김정선 ( Kim Jung-Sun )
울산대학교 의과대학 서울아산병원 진단병리학교실
유창식 ( Yu Chang-Sik )
울산대학교 의과대학 외과학교실
김진천 ( Kim Jin-Cheon )
울산대학교 의과대학 외과학교실
KMID : 0356720030190010020
Abstract
Purpose: We investigated whether the loss of E-cadherin function was related to the peritoneal seeding in colorectal carcinomas.
Methods: Eleven patients who had undergone a palliative resection for a colorectal carcinoma, with peritoneal seeding, were enrolled onto the study. The primary tumors and seeding nodules were analyzed with regarded to mutations in the expressions of the CDH1 and protein of E-cadherin using SSCP, direct sequencing and immunohistochemical staining.
Results: In the primary tumors, the E-cadherin was normally expressed in 9 of the 11 cases, with 2 cases showing a reduced expression. In the seeding nodules, the E-cadherin was normally expressed in 6 of the 11 cases, with 5 cases showing a reduced expression. The degree of E-cadherin expression in the seeding nodules was significantly decreased comparing to that in the primary tumors (P＜0.001). In the mutational analysis, there were no pathogenic mutations in either the primary tumors or the seeding nodules, with the exception of two silent changes in the ctgggt＞ctaggt (intron 2) and GTG＞GTA (codon 782).
Conclusion: The loss of E-cadherin expression might be related to peritoneal seeding. The functional derangement of E-cadherin in peritoneal seeding could possibly be caused by a mechanism, such as promoter methylation, rather than the mutation of the CDH1.
키워드
대장암;E-cadherin 복막전이
CDH1;Colorectal carcinoma;E-cadherin Peritoneal seeding
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