Sulindac에 의한 대장암 세포주 HT-29 세포의 세포사멸 기작 연구-미토콘드리아 통로를 중심으로
Sulindac-induced Apoptosis without Oligonucleosomal DNA Fragmentation in HT-29 Cells-Its Special References to Mitochondrial Pathway -
박기재, 권육, 김성흔, 김민찬, 최홍조, 김영훈, 조세헌, 정갑중, 김성현, 권혁찬,
소속 상세정보
박기재 ( Park Ki-Jae )
동아대학교 의과대학 외과학교실
권육 ( Kwon Yuk )
동아대학교 의과대학 외과학교실
김성흔 ( Kim Sung-Heun )
동아대학교 의과대학 외과학교실
김민찬 ( Kim Min-Chan )
동아대학교 의과대학 외과학교실
최홍조 ( Choi Hong-Jo )
동아대학교 의과대학 외과학교실
김영훈 ( Kim Young-Hoon )
동아대학교 의과대학 외과학교실
조세헌 ( Joh Se-Heon )
동아대학교 의과대학 외과학교실
정갑중 ( Jung Ghap-Joong )
동아대학교 의과대학 외과학교실
김성현 ( Kim Sung-Hyun )
동아대학교 의과대학 내과학교실
권혁찬 ( Kwon Hyuk-Chan )
동아대학교 의과대학 내과학교실
KMID : 0356720040200040191
Abstract
Purpose: This study was undertaken to reveal the molecular mechanism underlying sulindac-induced apoptosis in the human colon cancer cell line HT-29 (mutant p53).
Methods: Apoptosis was determined by using Hoechst 33342 staining, and translocation of proteins was established by using immunofluorescence, immunoelectron microscopy, and Western blotting after ultra- fractionation.
Results: This type of apoptosis was associated with decreased mitochondrial membrane potential, a translocation of the apoptosis-inducing factor (AIF) to the nucleus, and morphological evidence of nuclear condensation. However, DNA electrophoresis did not elucidate the ladder pattern of DNA fragments. Instead, a pulse-field gel electrophoresis showed that sulindac led to disintegration of nuclear DNA into-high- molecular-weight DNA fragments of about 100～300 kbp.
Conclusions: Our findings indicate that sulindac induces large-scale DNA fragmentation, suggesting a predominantly AIF-mediated cell-death process, through translocation of the AIF to the nucleus in HT-29 cells. J Korean Soc Coloproctol 2004;20:191-198
키워드
세포사멸;대장암세포주 HT-29; 세포사멸 유발인자;고분자 DNA조각
Apoptosis;Colon cancer cell line HT-29;AIF;HMW DNA fragmentation
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