대장암에서 EGFR 검출의 방법론적 차이에 대한 비교연구
Methodologic Evaluation of EGFR Expression in Colorectal Cancer
오수연, 윤세진, 천승희, 이령아, 강보영, 이시내, 정순섭, 김광호,
소속 상세정보
오수연 ( Oh Soo-Yoon )
건국대학교 의과대학 외과학교실
윤세진 ( Yoon Se-Jin )
이화여자대학교 의과대학 외과학교실
천승희 ( Cheon Seung-Hui )
이화여자대학교 의과대학 외과학교실
이령아 ( Lee Ryung-Ah )
이화여자대학교 의과대학 외과학교실
강보영 ( Kang Bo-Young )
이화여자대학교 의과대학 의과학연구소
이시내 ( Lee Shi-Nae )
이화여자대학교 의과대학 병리학교실
정순섭 ( Chung Soon-Sup )
이화여자대학교 의과대학 외과학교실
김광호 ( Kim Kwang-Ho )
이화여자대학교 의과대학 외과학교실
KMID : 0356720060220020075
Abstract
Purpose: The epidermal growth factor receptor (EGFR) is a one of the transmembrane receptor proteins that play an important role in initiating tumor cell signaling and growth and is regarded as a promising target for cancer therapy. The EGFR expression rate has been reported to vary according to the detection method. The aims of this study were to evaluate the EGFR expression rate of a colorectal carcinoma by using immunohistochemical staining (IHC) and semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) and to analyze the correlation between these methods.
Methods: EGFR expression was investigated in tissue sections from 33 patients with a colorectal adenocarcinoma by using IHC and semiquantitative RT-PCR. IHC was performed with antibodies in a 1：40 dilution and a 1：80 dilution. The results of the three detection methods were compared with one another.
Results: The mean age of the patients was 61.9±12.2 years, and the male-to-female ratio was 1.2：1. The EGFR expression rates were 93.9% (31/33) in IHC with a 1：40 dilution, 87.9% (29/33) in IHC with a 1：80 dilution, and 66.7% (22/33) in RT-PCR. The result of IHC with a 1：40 dilution significantly correlated with the result of IHC with a 1：80
dilution (Pearson correlation 0.684, P＜0.01). There was no correlation between semiquantitative RT-PCR and IHC (1：40 dilution, 1：80 dilution).
Conclusions: The EGFR expression obtained by using IHC was consistent with different antibody dilutions. The expression rate obtained by using RT-PCR was significantly lower than that obtained by using IHC, and there was no statistical correlation between the expressions of EGFR obtained by using RT-PCR and IHC. A standardization for EGFR detection methods is needed to draw any conclusion concerning their activity in colorectal cancer. J Korean Soc Coloproctol 2006;22:75-80
키워드
면역조직화학염색;반정량적 중합 효소 연쇄반응;대장암
Epidermal growth factor receptor (EGFR);Immunohistochemistry;Semiquantitative RT-PCR;Colorectal cancer;EGFR
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