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Effect of Ionizing Radiation on Homotypic Cell Adhesion, Cell-Matrix Adhesion, Matrix Metalloproteinases Excretion of High Mucin Producing HM7 Colon Cancer Cells
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±èÇý°æ ( Kim Hye-Gyong )
Ãæ³²´ëÇб³ ÀÇ°ú´ëÇÐ ¿Ü°úÇб³½Ç
±èŵ¿ ( Kim Tae-Dong )
Ãæ³²´ëÇб³ ÀÇ°ú´ëÇÐ ¿Ü°úÇб³½Ç
¸®Çõ ( Li Ge )
Ãæ³²´ëÇб³ ÀÇ°ú´ëÇÐ ¿Ü°úÇб³½Ç
À±¿ÏÈñ ( Yoon Wan-Hee )
Ãæ³²´ëÇб³ ÀÇ°ú´ëÇÐ ¿Ü°úÇб³½Ç
KMID : 0356720060220030153
Abstract
Purpose: To investigate the effect of ionizing radiation on high mucin-producing colon cancer cells, we evaluated homotypic cell adhesion, cell-matrix adhesion, and matrix metalloproteinases (MMPs) on HM7 cells.
Methods: After an irradiation of 60 Gy for 48 hours on HM7 cells, we evaluated cellular proliferation, colony-forming ability, homotypic adhesion, cell-matrix binding, and integrin subunit expressions. Also, alterations of MMPs expression were analyzed by using zymography.
Results: Cell proliferation of HM7 colon cancer cells was not remarkably affected even after high doses of radiation; however, clonogenic cell growth was significantly affected. Homotypic cell-cell adhesion and cell adhesion to ECM components and basement membrane protein matrigel were significantly increased after irradiation. Radiation induced expressions of cell surface integrin ¥á2, ¥á3, and ¥â1 subunits of HM7 cells. The activities of secreted MMPs (MMP-9 and MMP-2) were remarkably inhibited by radiation.
Conclusions: These finding suggest the biologic characteristics of high-mucin-producing colorectal carcinomas. Even though the radiation- associated cellular alterations of HM7 cells with or without matrix proteins were not remarkably different from other cancer cell types studied, the radio-resistant behavior of high mucin producing HM7 cells may explain the aggressive characteristics of mucinous colorectal carcinomas. J Korean Soc Coloproctol 2006;22:153-161
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MMPs;Colon cancer;Radiation;Adhesion molecule
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