간전이를 동반한 대장암 환자에서 cDNA Microarray Chip을 이용한 유전자 발현 양상 비교 연구의 초기 결과
Differential Expressions of Genes in Colorectal Cancer with Liver Metastasis by using a cDNA Microarray Chip: A Pilot Study
김재오, 최규석, 김상걸, 장은정, 김우리, 이인택, 전수한, 김문규, 김정철,
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김재오 ( Kim Jae-O )
포천중문의과대학교 구미차병원 외과학교실
최규석 ( Choi Gyu-Seog )
경북대학교 의과대학 외과학교실
김상걸 ( Kim Sang-Geol )
경북대학교 의과대학 외과학교실
장은정 ( Chang Eun-Jung )
경북대학교 의과대학 면역학교실
김우리 ( Kim Woo-Ri )
경북대학교병원 생명의학연구소
이인택 ( Lee In-Taek )
경북대학교 의과대학 외과학교실
전수한 ( Jun Soo-Han )
경북대학교 의과대학 외과학교실
김문규 ( Kim Mun-Kyu )
경북대학교 의과대학 면역학교실
김정철 ( Kim Jeong-Chul )
경북대학교 의과대학 면역학교실
KMID : 0356720070230020116
Abstract
Purpose: Liver metastasis is the most common type of failure in the treatment of colorectal cancer. The identification of differential expressions of genes in colorectal cancer and liver metastasis is important to differentiate the genetic mechanism of carcinogenesis and liver metastasis from that of a normal mucosa. The aim of this study is to find candidate genes playing roles in liver metastasis of colorectal cancer by using cDNA microarray.
Methods: We screened a group of genes differentially expressed in a normal mucosa and in cancer and liver metastasis by using a 4.7 K cDNA microarray chip in 8 patients with far advanced colorectal cancer from Jan 2003 to May 2004 at Kyungpook National University Hospital.
Results: A comparison of mRNA expressions of genes in normal mucosa vs. cancer, normal mucosa vs. liver metastasis, and cancer vs. liver metastasis, 76 and 27 known and unknown genes were significantly over-expressed in cancer and liver metastasis, respectively. Also 62 and 26 genes were down- regulated in cancer and liver metastasis. Among those genes, TIMP-1, SRY-box9, Rattus norvegicus fibronectin 1, mitotic check point regulator, etc. were constantly up- regulated in cancer or metastasis, and hsgk, etc. were down-regulated in cancer or liver metastasis.
Conclusion: The cDNA microarray chip technique could be a useful for robust screening of candidate genes involved in carcinogenesis or metastasis of colorectal cancer. J Korean Soc Coloproctol 2007;23:116-128
키워드
대장암;간전이;유전자 표현;cDNA 미세배열 칩
Colorectal cancer;Liver metastasis;Gene expression;cDNA microarray chip
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