냉적응 생쥐의대식세포 활성화에 미치는 PKC 억제제와 Ca^(2+) Antagonist의 영향
Effects of PKC inhibitors and Ca^(2+) Antagonists on the Activation of Macrophages in the Cold Adapted Mice
정노팔, 최인호, 오영근, 박한우, 정일형,
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정노팔 ( )
연세대학교 이과대학 생물학과
최인호 ( )
연세대학교 문리대학 생명과학과
오영근 ( )
연세대학교 문리대학 생명과학과
박한우 ( )
연세대학교 이과대학 생물학과
정일형 ( )
한림대학교 의과대학 생리학교실
KMID : 0357119950170030161
Abstract
Human thymus 조직으로부터 cDNA library를 만들었다. 무작위로 선별한 cDNA clone중에서 468개 염기로 된 cDNA clone (PTH209)을 검색한 결과 124개의 아미노산을 code하는 EAP(Epstein-Barr asseciated protein) 유전자임을 확인하였다. Thymus의 EAP와 Epstein-Barr 바이러스 positive 및 negative cell line의 RNP에서 분리한 EAP^(13)를 비교한 결과, coding region은 동일하였으나 3´UT region은 달랐다. 더구나 EBERs(Epstein-Barr encoded RNAs) 와 La 자가항원이 결합된 RNP의 EAP와 마찬가지로, thymus의 EAP는 sea urchin의 developmental stage-specific 단백질로 알려진 217^(2) 과 매우 유사한 아미노산 서열을 보였다. 이런 결과로 미루어 thymus의 EAP는 thymic development에 중요한 역할을 하는 것으로 사료된다.
To study the adaptation of immune systems in the animals kept in cold temperature, the recovery rate of peritoneal exudated mouse macrophage was measured in mouse bred at room temperature(RT) or at cold(S±2 C). NBT-reduction activities and phagocytic activities were measured too, to the macrophages recovered. The results are as follows; More than 40% of cells were recovered in the cold treated groups compaired to the RT bred groups. The NBT-reduction activities were totally blocked at 10mM EGTA, and were below 10% of the control in H7 100pM and TMB-8 0.1mM. The NBT-reduction activity of cold treated groups were higher than RT group at lower concentration than H7100pM and TMB-8 0.1mM . The H7 resulted no changes in phagocytic activities both cold and RT groups at 0.1-100pM. The TMB-8 resulted in significant decrease in phagocytic activities at 0.1 mM and 1mM. The conclusions are 1) the cold treated animals showed an increase in the NBT-reduction activities and in the macrophage recovery rates, 2) H7 was unable to block phagocytosis of yeasts up to 10011M concentration, 3) lectin-like yeast cell surface molecules have different signaling mechanism form IgG molecules.
Macrophages;Phagocytosis;Nitrobluetetrazolium;1-(5-isoguinolinylsulfonyl)-2-methylpiperazine(H-7);3,4,5-trimethoxybenzoic acid 8-(diethylamino)octylester (TMB-8).
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