Glucose Oxidase를 利用한 血糖의 定量法에 關하여
The Determination of Blood Sugar by Glucose Oxidate Method
소속 상세정보
김재식(金在植)/Jae Sik Kim
윤성직(尹聖稷)/박희숙(朴熙淑)/박준식(朴俊植)/오복득(吳福得)/Seong Jik Yoon/Hi Sook Park./Joon Shik Park/Bok Duk Oh
KMID : 0357919690030010009
Abstract
Since high degree of specificity of glucose oxidase for beta-glucose had been
demonstrated, the use of the enzyme, glucose oxidase and peroxidase coupled with a
chromogenic oxygen acceptor such as O-dianisidine for the colorimetric determination of
glucose in body fluid was proposed from several investigators. In this report the glucose
level was determined in the non-and deproteinized serum and blood by means of
glucose oxidase method of the Sigma Company, also the recovery rate was examined by
addition of the standard glucose compatible to 50 to 100mg% with following results :
1. It was found that this method was available in the determination of blood sugar
without deprateinization in nonhemolytic serum.
2. When the known amounts of glucose solution were added to the serum and blood,
the recoveries were ranged from 90 to 110%.
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