순수 임파구 분리에 관한연구
Separation of Lymphocytes from Human Peripheral Blood
이귀녕, 김홍기,
소속 상세정보
이귀녕 ( )
연세대학교 의과대학 임상병리과
김홍기 ( )
서울대학교 의과대학 임상병리과
KMID : 0357919750090010055
Abstract
This paper describes a technique for the isolation of lymphocytes from haparinized
human peripheral blood. When whole blood was layered on top of a mixture of Ficoll
and Isopaque and gradient centrifuged at room temperature, the cellular elements of
whole blood were divided into two main parts, a lymphocyte bone in plasma layer and a
layer consisting of sedimented granulocytes and erythrocytes at the bottom of the tube.
By this method the isolation of viable lymphocyte with 96.6% purity was possible in a
short period of time. Impurities found in the lymphocyte zone was granulocytes; 0.2%,
monocytes; 2.8%, eosinophils; 0.4%, erythrocytes; less than 5% of total cell counts
respectively.
Isolating conditions and purities together with detailed directions are presented. It is
shown in the present paper that adopting the procedure introduced a lymphocyte
suspension of extreme purity with high viability may be obtained for lymphocyte
research.
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