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The Influence of Technical Factors on NBF test
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KMID : 0357919750090020181
Abstract
The results of NBT test were found very depending on how the testis performed. NBT
tests performed simultaneously on various concentration of heparin, various length of
incubation time, different mixing instruments, venous blood and capillary blood, and
scoring of three good trained technicians, from 90 healthy individuals.
Heparin increased proportions of NBT positive neutrophils and lowest possible
concentration should be used. NBT tests showed a considerably higher proportion of
NBT positive neutrophils in capillary blood than in venous blood. 15 minutes incubation
time at 37¡É affected unstable and obscure formazan deposits on NBT positive
neutrophils than 25 minutes. Scoring of different good trained technicians are the same
results on NBT positive neutrophils.
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