흰쥐 膵臟 外分泌 細胞의 走査電子 顯微鏡的 觀察
Scanning Electron Microscopy of the Ultrastructure of Pancreatic Acinar Cells
소속 상세정보
권건영(權建營)/Kun Young Kwon
곽정식(郭精植)/손태중(孫泰重)/Jeong Sik Kwak/Tae Joong Sohn
KMID : 0357919830170010046
Abstract
要 約
저자는 췌장 외분비 세포의 순기관의 표면 구조를 Osmium-DMSO-Osmium법을 이용하
여 주사전자현미경으로 관찰하고 이와 아울러 광학현미경 및 투과전자 현미경 소견과 비교
하여 보고 얻은 성적을 요약하면 다음과 같다.
Osmium-DMSO-Osmium법으로 췌장 외분비세포의 소기관을 분명하게 부출할 수 있었
다.
광학현미경적으로 잘 볼 수 없었던 세포내 미세구조들을 투과형 전자현미경으로 상세하게
관찰할 수 있었다.
주사전자현미경으로 관찰된 췌장 외분비세포의 소기관 즉 분비과립, RER, 핵, Golgi장치,
mitochondria등은 투과형 전자현미경의 소견과 같이 RER은 층상으로, 핵표면은 매끈하게,
Golgi장치는 vesicle, lamella, vacuole등 3요소로 그리고 mitochondria는 원형 또는 타원형으
로서 그 표면은 매끈하며 많은 cristae 를 갖고 있었다.
상기한 각 소기관의 내면들도 모두 대체로 약간의 과립상의 구조를 정하였다.
#초록#
Several methods of tissue preparation and different modes of operation of the scanning
electron microscope were used to study the ultrastructure of cells. The author examined
ultrastructure of rat pancreatic acinar cells prepared by Osmium-DMSO-Osmium and
subsequent cracked with razor blade, and discussed the scanning electron microscope
images in comparison with light microscopic and transmission electron microscopic
findings. The results were as follow.
The ultrastructure of pancreatic acinar cells prepared by Osmium-DMSO-Osmium
method was well exposed.
The transmission electron microscopy easily revealed the cellular organelles which
were poorly visualized by light microscope.
The ultrastructures of pancreatic acinar cells studied by SEM were well correlated
with results obtained by TEM. The surface of nuclear membrane was smooth.
Mitochondria were round or elongated and had many cristae. RER arranged in lamellae
Pattern with occasional dilatation. Golgi complex was composed of vesicles, lamellae and
vacuoles. The inner surfaces of above mentioned organelles revealed relatively granular
textures. One important point revealed by cracked samples concerns the findings of
spherical secretory granules within thin walled cavities, which suggestive of Golgj
vesicle origin. These vesicles appear connected by small openings. It is suggested that
they may represent a system of intercommunicating chambers(vacuoles).
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