白鼠膵管結紮後 腺房細布萎縮의 機轉
Mechanisms of Acinar Cell Deletion in Rat Pancreas Following Experimental Duct Ligation
소속 상세정보
김상표(金相豹)/Sang Pyo Kim
권건영(權建榮)/이상숙(李相淑)/정재홍(鄭在泓)/Kun Young Kwon/Sang Sook Lee/Chai Hong Chung
KMID : 0357919890230010051
Abstract
요 약
췌관의 결찰후에 생기는 췌장위축의 기전을 규명하고자 영양상태가 양호한 평균체중 200
gm정도의 Sprague-Dawley종 백서 57마리를 실험에 사용하였다. 실험동물들은 췌관의 부분
결찰을 시행한 뒤, 술후 1, 3, 6, 12시간, 1, 2, 3, 4, 5일, 및 1, 2, 8주에 각각 도살하여 광학
현미경 및 전자현미경적으로 검색을 시행하여 다음과 같은 성적을 얻었다.
1) 광학현미경적 소견으로는, 초기소견으로 간질성 부종 및 국소적 괴사가 2일째까지 관
찰되고, 또 이 시기에 선방세포내의 zymogen granule의 감소, 세포질내의 공포형성 및 핵농
축과 분절을 보이고, 선방세포의 수가 감소되고, 그리고 선방세포질내에 apoptoticbody가 출
현하였다. 5일째에는 선방세포가 소실되고 그 이후에는 개재소관들 (intercalated ducts)만
남았다.
2) 투과 및 주사 전자현미경적 소견으로는, 선방세포 내에 membrane-bounded apoptotic
body가 췌관결찰후 1일째 부터 출현하여 3일째에 가장 현저한 증가를 보였다. 이들
apoptotic body는 대부분 인접한 대식세포나 혹은 상피세포에 의해 탐식되어 있었다.
이상의 성적으로 미루어보아 췌관결찰 후에 오는 선방세포의 위축은 apoptosis로 인한 선
방세포의 결실(deletion)에 의한 것으로 사료된다.
#초록#
This study was carried out to investigate the mechanisms of acinar cell deletion,
leading to the pancreatic atrophy of rat pancreas after experimental duct ligation.
Fifty-seven male Sprague-Dawley rats, maintained on a stock diet, weighing 200 gm, in
average, were divided into 2 experimental groups.
Group 1. Control group. Six rats. Abdominal cavity was opened and closed without
further treatment.
Group 2. Fifty-one rats. Animals were treated with partial ligation of the pancreatic
ducts according to the procedure developed by Hultquist followed by sequential sacrifices
at: 1 hour (3 rats), 3 hours (3 rats), 6 hours (6 rats), 12 hours (3 rats) and 24 hours (8
rats); 2 days (8 rats), 3 days (3 rats), 4 days (3 rats) and 5 days (5 rats); 1 week (3
rats), 2 weeks (3 rats) and 8 weeks (3 rats); after ligation. Distal part of the pancreas
of the animals after partial ligation was extirpated and examined by both light and
electron michoscopy.
The results obtained were as follows:
Light microscopically, noted were an interstitial edema and focal necrosis of the
pancreatic tissue along with fine vacuolization and depletion of the zymogen granules in
the acinar cell cytoplasms and condensation of the acinar cell nucleus. These changes
were observed by 2 days after ligation. At about the same time, one can observe the
dense body, identified to be apoptotic body, in the acinar cells which were found to be
decreased in quantity. By 5 days after ligation, no recognizable acinar cells left in the
collagenous stroma except intercalated ducts. Conspicuous stroma except intercalated
ducts. Conspicuous stromal hyalinization, thereafter
Electron microscopically (TEM and SEM), nuclear condensation and margination
toward the nuclear membrane was noted by 6 hours after duct ligation. By 24 hours
sporadic membrane-bounded apoptotic bodies appeared in the acinar cells, the number of
which reach. ins to the peak by 3 days after ligation. These apoptotic bodies were found
to be phagocytes by either intraepithelial mononuclear phagocytes or adjoining acinar
cells.
It can be concluded, therefore:
That orderly remodeling of pancreatic exocrine tissue during atrophy is effected by
rapid deletion of acinar cells by apoptosis.
키워드
Apoptosis; Atrophy; Pancreatic duct ligation;
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