면역전자현미경 검색에 의한 뉴우모시스티스 카리니 표면 항원 물질의 발현
Expression of Antigenic Surface Molecules of Pneumocystis Carinii by Immunoelectron Microscopic Examination
소속 상세정보
권건영/Kwon KY
조승제/김상표/박관규/장은숙/Cho SC/Kim SP/Park KK/Chang ES
KMID : 0357919980320060393
Abstract
This study was carried out to investigate the morphologic characteristics and localization
of antigentic molecules of Pneumocystis carinii in experimentally induced P. carinii in
rats. After six weeks of administration of low protien diet and dexamethasone,
Sprague-Dawley rats were sacrificed to submit Lungs or bronchoalveolar lavage for the
study. Monoclonal (092, 900, 902, and 904) and polyclonal (SP-D) antibodies were for
immunohistochemistry and immunoelectron microscopy (ITEM and ISEM).
Immunohistochemically P. Carinii organisms were well identified as clusters or separated
forms in the alveolar spaces being frequently attached to the attached to the alveoar
walls. Immunoelectron microscopically the adherence of gold particles of gold particles
were observed on the surface of all stages of the P. carinii. Occasionally positive
immunogold labeling was observed in the cytoplasm of the trophozoites and on the
pellicle of the intracytic bodies within the cysts. The monoclonal antibobies 092, 900,
902, and 904 reacted mainly with pellicles of P. carinii, whereas SP-D labeled on the
pellicles, intracystic bodies, stopa는 of the alveolar macrophage, and free floated
surfactant material on the alveolar spaces. The immunogold particles were observed
more diffusely and intensely in the systs than in the trophozoites. These results indicate
that antigen is mainly localized on the pellicles, and accumulated during development
from the trophosoite to the cyst stages.(Korean J pathol 1998; 32: 393∼403)
키워드
Pneumocystis carinii; Electron microscopy; Immunohistochemistry; Immunoelectron microscopy;
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