뉴우모시스티스 카리니 폐렴에서 폐포 대식세포와 뉴우모시스티스 카리니의 상호 반응에 관련된 Surfactant-D 단백질 및 TNF-α의 발현
Expression of Surfactant-D Protein and TNF-α in the Interaction of Pneumocystis Carinii and Alveolar Macrophages in Pneumocystis Carinii Pneumonia
권건영, 박관규, 박창권, 전영준, 장은숙,
소속 상세정보
권건영 ( Kwon Kun-Young )
계명대학교 의과대학 병리학교실
박관규 ( Park Kwan-Kyu )
계명대학교 의과대학 병리학교실
박창권 ( Park Chang-Kwon )
계명대학교 의과대학 흉부외과학교실
전영준 ( Jeon Young-June )
계명대학교 의과대학 내과학교실
장은숙 ( Chang Eun-Sook )
계명대학교 의과대학 병리학교실
KMID : 0357919990330090684
Abstract
Alveolar macrophages participate in the host defense against P. carinii, but the mechanisms in degradation and clearance of the organism from lung has not been well established. We observed the transmission and scanning electron microscopic features and evaluated the expression of TNF-α and Surfactant-D in the interaction of P. carinii with alveolar macrophages. Expression of TNF-α and Surfactant-D in the experimentally induced P. carinii pneumonia in rat was examined by immunohistochemistry and immunoelectron microscopy. Electron microscopically, the alveolar macrophages phagocytized trophozoites and cysts of P. carinii micro-organisms. Immunohistochemically TNF-α was strongly expressed in the cytoplasms of alveolar macrophages. Postembedding immunogold labeling for Surfactant-D protein was expressed on the pellicles of trophozoites and cysts, P. carinii micro-organisms in the cytoplasms of macrophages, free floating surfactant materials and multilamellar bodies of type II epithelial cells. We conclude that alveolar macrophages interacted with P. carinii micro-organisms respond with increased expression of TNF-α. TNF-α may bind to P. carinii and exert a direct toxic effect upon the micro-organisms. Surfactant-D protein may augment binding of P. carinii to the alveolar macrophages and enhance the clearance of the micro-organisms.
키워드
Pneumocystis carinii;Alveolar macrophages;TNF-α;Surfactant-D;Immunogold labeling
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