악성림프종에서 Phospholipase C-γ1와 γ2의 발현
Expression of Phospholipase C-γ1 and γ2 in Non-Hodgkin’s and Hodgkin’s Lymphoma
엄대운, 김성숙, 우영주, 서재희, 허주령, 백애란, 김재호, 류성호, 서판길,
소속 상세정보
엄대운 ( Eom Dae-Woon )
울산대학교 의과대학 병리학교실
김성숙 ( Kim Sung-Sook )
울산대학교 의과대학 병리학교실
우영주 ( Woo Yeong-Ju )
울산대학교 의과대학 병리학교실
서재희 ( Suh Jae-Hee )
울산대학교 의과대학 병리학교실
허주령 ( Huh Joo-Ryung )
울산대학교 의과대학 병리학교실
백애란 ( Paik Ae-Ran )
포항성모병원 해부병리과
김재호 ( Kim Jae-Ho )
포항공과대학교 생명과학과
류성호 ( Ryu Sung-Ho )
포항공과대학교 생명과학과
서판길 ( Suh Pan-Ghil )
포항공과대학교 생명과학과
KMID : 0357920000340020113
Abstract
Phospholipase C (PLC) plays a role in ligand-mediated signal transduction for cellular activity such as proliferation and differentiation. A recent observation that PLC-γ1 is highly expressed in some kinds of human cancer tissue supports the view that PLC-γ1 may be involved in proliferation and carcinogenesis. PLC-γ2 is known to be involved in B cell differentiation and maturation. However, there have been few studies about the expressions of PLC-γ1 and γ2 in human lymphoid malignancy. In the present study, we examined the contents of PLC-γ1 and γ2 in 10 cases of B cell, 10 cases of T cell non-Hodgkin’s lymphoma and 5 cases of Hodgkin’s lymphoma to find out whether these enzymes play any role in the carcinogenesis by immunohistochemistry and immunoprecipitation. Immunoprecipitation analysis revealed that in contrast to increased expression of PLC-γ2 only in B cell lymphoma, a considerably higher level of PLC-γ1 was detected in both B and T cell lymphoma. Immunohistochemical finding confirmed this observation. PLC-γ1 and PLC-γ2 were expressed in the cytoplasm of most tumor cells. PLC-γ2 was also expressed in mature B cells, while PLC-γ1 was not expressed in reactive non-tumor cells. These results suggest that PLC-γ1 mediated signal transduction implicates a significant role in the carcinogenesis of all types of lymphoid tissue, and PLC-γ2 may play a role in the carcinogenesis of B cell lymphoma as well as B cell differentiation.
키워드
PLC-γ1;PLC-γ2;Non-Hodgkin’s lymphoma;Hodgkin’s lymphoma
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