백서 뇌에서 망간에 의한 기저핵의 자멸사 및 병리조직학적 변화
Histopathologic Change and Apoptotic Profile in Basal Ganglia of Rat Induced by Manganese Administration
하창원, 이종임, 김정란, 장석용, 김기권, 김동훈,
소속 상세정보
하창원 ( Ha Chang-Won )
동국대학교 의과대학 병리학교실
이종임 ( Lee Jong-Im )
동국대학교 의과대학 병리학교실
김정란 ( Kim Jung-Ran )
동국대학교 의과대학 병리학교실
장석용 ( Jang Suk-Yong )
동국대학교 의과대학 병리학교실
김기권 ( Kim Ki-Kwon )
동국대학교 의과대학 병리학교실
김동훈 ( Kim Dong-Hoon )
동국대학교 의과대학 병리학교실
KMID : 0357920000340060419
Abstract
Mn (manganese) is known to induce Parkinsonian neurological disorder. Several lines of evidence suggest that apoptosis is involved not only in physiological cell death during normal development but also in neurodegenerative disease. The mechanism of Mn induced cell death remains poorly understood. In the present study, we evaluated the morphologic changes and apoptotic profile in basal ganglia using rat model of Mn toxicity. The rats were divided into three groups: the first group was a control; the second group was subdivided by administration dosage of Mn into group A (5, 10 mg MnC12/ kg) and group B (20, 40 mg MnC12/kg). The rats of each subgroup received a injection of Mn via tail vein every week for 4 weeks. The second group received 4 repeated injection of 10 mg MnC12/kg in the same manner and the rats were sacrificed at day 1, 3 & 7 in group I and at day 10, 21, 42, and 90 in group II after the last injection. A significant loss of neuron and gliosis were observed in the basal ganglia in the experimental groups (p＜0.05), which were more pronounced in group II than in the control or group I. No significant difference in number of nerve cells or degree of gliosis was identified in the substantia nigra. Apoptotic cells were also increased in basal ganglia of experimental groups and appeared among neurons (10%), glial cells (10%), and endothelial cells (60%). Apoptotic figures were consistently noted through the entire experimental period after Mn injection in basal ganglia. In conclusion, these results demonstrate that Mn-induced cytopathic insult affects various cell types in basal ganglia and shows variable sensitivity in the different regions of brain, especially in the apoptotic cell death of the neuron. The overaccumulation of Mn in the brain might be attributed from the breakdown of blood-brain barrier due to the injury through the apoptosis.
키워드
Rat;Brain; Mn (manganese);Apoptosis;Blood-brain barrier
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