뉴우모시스티스 카리니 폐렴에서 폐포상피세포와 뉴우모시스티스 카리니의 상호반응에 관련된 Fibronectin, Virtonectin 및 Surfactant-A, D의 발현
Expression of Fibronectin, Vitronectin, Surfactant-A and D in Interaction of Pneumocystis carinii and Alveolar Epithelial Cells in Pneumocystis carinii Pneumonia
권건영, 전영준, 장은숙,
소속 상세정보
권건영 ( Kwon Kun-Young )
계명대학교 의과대학 병리학교실
전영준 ( Jeon Young-June )
계명대학교 의과대학 내과학교실
장은숙 ( Chang Eun-Sook )
계명대학교 의과대학 병리학교실
KMID : 0357920000340090625
Abstract
Both fibronectin and vitronectin bind to Pneumocystis carinii (P. carinii) and mediate the attachment of the organisms to respiratory epithelial cells. Surfactant A and D play a role in the interaction between P. carinii and host cells. In this study we examined the expression of fibronectin, vitronectin, surfactant-A and D in the interaction between P. carinii and alveolar epithelial cells by immunohistochemistry and pre-embedding immunoelectron microscopy. The experimental rat model of P. carinii pneumonia was induced by administration of low protein diet (8%) and drinking water containing dexamethasone (2 mg/liter) for 6 to 8 weeks. The primary antibodies for light and electron microscopic immunohistochemistries were monoclonal antibodies including fibronectin (1：100) and vitronectin (1：100), and polyclonal antibodies including surfactant A (1：50) and D (1：50), respectively. Light microscopic immunohistochemistry for the fibronectin, vitronectin, surfactant-A and D showed strong expressions on the P. carinii and surface linings of type I alveolar epithelial cells. The electron microscopic immunohistochemistry of the fibronectin and vitronectin showed a strong immunoexpression along the surface pellicles and tubular extensions of P. carinii trophozoites, and surface membranes of the type I epithelial cells. The surfactant-A and D proteins showed a strong expression on the pellicles of P. carinii and surface membranes of the type I epithelial cells, but a weak expression on the free-floating surfactant materials. In conclusions, the trophozoites of P. carinii were mostly attached to type I epithelial cells. The fibronectin, vitronectin, surfactant-A and D were strongly expressed, and played an enhancing role in the binding between the P. carinii organisms and the type I alveolar epithelial cells.
키워드
Pneumocystis carinii;Type I epithelial cells;Fibronectin;Vitronectin;Surfactant-A and D
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