Cyclosporine 투여 후 흰쥐에 발생한 신병증에서 Transforming Growth Factor-β1의 발현
Expression of Transforming Growth Factor-β1 in Cyclosporine-Induced Nephropathy in Rats
강유나, 박관규, 황미열, 권건영, 이상숙, 장은숙, 김현철,
소속 상세정보
강유나 ( Kang Yu-Na )
계명대학교 의과대학 병리학교실
박관규 ( Park Kwan-Kyu )
계명대학교 의과대학 병리학교실
황미열 ( Hwang Mee-Yul )
계명대학교 의과대학 병리학교실
권건영 ( Kwon Kun-Young )
계명대학교 의과대학 병리학교실
이상숙 ( Lee Sang-Sook )
계명대학교 의과대학 병리학교실
장은숙 ( Chang Eun-Sook )
계명대학교 의과대학 병리학교실
김현철 ( Kim Hyun-Chul )
계명대학교 의과대학 내과학교실
KMID : 0357920000340090642
Abstract
Cyclosporine nephropathy was induced by intraperitoneal injection of cyclosporine 25 mg/kg in Sprague-Dawley rats daily for 1, 4, 8, and 12 weeks to clarify the relationship between cyclosporine nephropathy and the expression of TGF-β1 with extracellular matrix deposition. On light microscopic examination, the kidneys in the 12 week cyclosporine-treated rats showed focal or striped fibrosis, vacuolization of tubular cells, and injury of endothelial cells. Immunohistochemically, TGF-β1 protein was strongly expressed in the cyclosporine-treated rat kidneys, especially in the glomerular endothelial cells, interstitial endothelial cells, tubular epithelial cells, and parietal cells in the Bowman’s capsule of the glomerulus as well as the periglomerular arterioles. The amount of TGF-β1 expression was correlated with the morphological change in the cyclosporine-treated rats. Extracellular matrix, such as fibronectin and collagen IV, was also expressed in the endothelial cells of the glomerulus and the interstitium. It can be concluded, therefore that TGF-β1 protein is probably involved in the early stage of fibrogenesis in cyclosporine nephropathy. It can be postulated that cyclosporine nephropathy results from the accumulation of extracellular matrix associated with the increase of TGF-β1 transcription. Therefore, these results could be used in reducing fibrosis in cyclosporine nephropathy.
키워드
Cyclosporine nephropathy;Fibrogenesis;TGF-β1 protein;Extracellular matrix
원문 및 링크아웃 정보
등재저널 정보