인체 갑상선질환에서 S100A6 유전자의 발현 양상
Expression Patterns of S100A6 Gene in Human Thyroid Diseases
김주헌, 김재화, 윤선영, 주종혁, 최인성, 박미자,
소속 상세정보
김주헌 ( Kim Ju-Hun )
을지대학교 의과대학 병리학교실
김재화 ( Kim Jae-Wha )
생명공학연구소
윤선영 ( Yoon Seon-Young )
생명공학연구소
주종혁 ( Joo Jong-Hyuck )
을지대학교 의과대학 병리학교실
최인성 ( Choi In-Seong )
생명공학연구소
박미자 ( Park Mee-Ja )
을지대학교 의과대학 병리학교실
KMID : 0357920000340110934
Abstract
S100A6 (calcyclin) is a member of the S100 family and has been originally isolated from the cDNA library of Syrian baby hamster kidney cells. The S100A6 gene expression is reported to remain high throughout the cell cycle following induction by serum or growth factors, suggesting that the gene may be required for cell cycle progression. Nevertheless, the role that S100A6 may play in tumor progression remains unknown. In this study, we have explored the expression patterns of S100A6 gene in human thyroid tissues by northern blot analysis. Using the S100A6 monoclonal antibody, we carried out the immunohistochemical staining to determine the distribution/localization of S100A6 protein within tumor or non-tumorous cells of the thyroid. To modulate the regulation of endogenously expressed S100A6 protein in the intracellular level, overexpressed or anti-sense treated transfectant was constructed by using the eukaryotic expression vector. As a result, immunohistochemistry for S100A6 showed a strong positivity in the malignant tumors of thyroid and a high expression level of S100A6 protein affected cell proliferation in the overexpressed transfectant. These findings suggest that S100A6 may be involved in the tumor pathogenesis and provides another parameter for the differentiation of malignant and benign lesions. A well defined monoclonal antibody against S100A6 protein is now available for the immunohistochemical studies of the various thyroid tissues.
키워드
S100A6;Monoclonal antibody;Thyroid
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