피부 T세포 증식성 병변의 클론성 확인
Molecular Diagnosis of Cutaneous T Cell Lymphoproliferative Diseases
박지영, 이명훈, 곽은경, 김동자, 박태인, 배한익,
소속 상세정보
박지영 ( Park Ji-Young )
경북대학교병원 해부병리과
이명훈 ( Lee Myung-Hoon )
경북대학교병원 해부병리과
곽은경 ( Kwak Eun-Kyung )
경북대학교병원 해부병리과
김동자 ( Kim Dong-Ja )
경북대학교병원 해부병리과
박태인 ( Park Tae-In )
경북대학교병원 해부병리과
배한익 ( Bae Han-Ik )
경북대학교병원 해부병리과
KMID : 0357920000340110941
Abstract
It is often problematic to diagnose T-cell lymphoproliferative disorders of the skin because of the difficulty in establishing clonality in paraffin-embedded tissue. We used polymerase chain reaction single strand conformational polymorphism (PCR-SSCP) and heteroduplex analysis in paraffin embedded tissue to detect clonal rearrangement of T-cell receptor γ (TCRγ) gene in 17 T-cell lymphoproliferative disorders and 6 atypical lymphoproliferative diseases. We used polymerase chain reaction to detect TCRβ gene rearrangement in 8 of 17 cases which did not show TCRγ gene rearrangement. Jurkat cell lines were used as monoclonal controls. DNA was extracted from 5 biopsies of T-cell lymphomas, 10 biopsies of mycosis fungoides, 2 biopsies of lymphomatoid papulosis, and 6 biopsies of atypical lymphoproliferative lesions. We detected monoclonality in 5 of 5 T-cell lymphoma cases, 2 of 2 lymphomatoid papulosis cases, 6 of 10 mycosis fungoides cases, and 2 of 6 atypical lymphoproliferative disease cases. We conclude that nonradioactive PCR-SSCP for TCR gene rearrangement analysis is a useful adjunct to routine histological and immunophenotypic methods in the diagnosis of cutaneous T cell lymphoproliferative disorders in paraffin embedded tissue.
키워드
Polymerase chain reaction;Single strand conformational polymorphism;Heteroduplex;T-cell receptor;Cutaneous T-cell lymphoproliferative diseases
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