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Molecular Cloning of Novel Genes Related to the Craniofacial Development of Human Embryo
이영준, 고택수, 한형욱, 이상신, 김은철, 김연숙, 이석근, 지제근,
소속 상세정보
이영준 ( Lee Young-Jun )
강릉대학교 치과대학 구강병리학교실
고택수 ( Go Tak-Soo )
강릉대학교 치과대학 구강병리학교실
한형욱 ( Han Hyung-Wook )
강릉대학교 치과대학 구강병리학교실
이상신 ( Lee Sang-Shin )
강릉대학교 치과대학 구강병리학교실
김은철 ( Kim Eun-Cheol )
원광대학교 치과대학 구강병리학교실
김연숙 ( Kim Yeon-Sook )
강릉대학교 치과대학 구강병리학교실
이석근 ( Lee Suk-Keun )
강릉대학교 치과대학 구강병리학교실
지제근 ( Chi Je-Geun )
서울대학교 의과대학 병리학교실
KMID : 0357920000340120961
Abstract
In order to obtain novel genes for craniofacial development of human, molecular cloning and sequencing were performed and followed by in situ hybridization in tissue sections. Subtracted cDNA library of craniofacial tissue from 8 weeks old human embryo was made by the subtraction with cDNA of RHEK cells. A total of 231 clones were obtained and their partial sequence data disclosed that 214 clones were nonredundant in Genebank search. We have done in situ hybridization screening on the craniofacial sections of a 10 weeks old human fetus, and found significant positive reaction in 30 clones. Depending on the cell type of similar developmental origin, the positive reactions could be divided into four groups: first group showed an intense positive reaction in neural tube, ganglion, and a part of peripheral nerve tissue, second group relatively diffuse positive reaction in neural tube, cartilage, epithelium, and muscle, third group localized positive reaction in nerve, and muscle, and fourth group positive reaction in almost all kinds of cells of craniofacial tissues. Although every clone showed different expression patterns in the craniofacial development, some of them showed intense mRNA expressions in the characteristic cell type. Because this study also aimed to test a screening methods to find out novel genes related to craniofacial development by the subtracted cDNA library and in situ hybridization, the intense positive reaction of a certain clone by in situ hybridization may indicate its role in the developmental processes. We presumed that 30 clones selected in this study are possibly important new genes for the development of human craniofacial structure.
키워드
Human embryo;Novel genes;Craniofacial;In situ hybridization
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