Hepatitis B Virus X 형질전환 생쥐와 인체 간세포암종에서 Proliferating Cell Nuclear Antigen 발현과 자멸사
A Study on the Expression of Proliferating Cell Nuclear Antigen and Apoptosis of theHepatocellular Carcinoma in Human and Hepatitis B Virus X Transgenic Mice
문형배, 유대열, 유형륜, 소병준, 채권묵, 김학철, 윤기중, 한원철, 조향정, 김보용,
소속 상세정보
문형배 ( Moon Hyung-Bae )
원광대학교 의과대학 병리학교실
유대열 ( Yu Dae-Yeul )
생명공학연구소
유형륜 ( Yoo Hyung-Ryun )
원광대학교 의과대학 병리학교실
소병준 ( So Byung-Joon )
원광대학교 의과대학 외과학교실
채권묵 ( Chae Kwon-Mook )
원광대학교 의과대학 외과학교실
김학철 ( Kim Haak-Cheol )
원광대학교 의과대학 내과학교실
윤기중 ( Yun Ki-Jung )
원광대학교 의과대학 병리학교실
한원철 ( Han Won-Cheol )
원광대학교 의과대학 병리학교실
조향정 ( Jo Hyang-Jeong )
원광대학교 의과대학 병리학교실
김보용 ( Kim Bo-Yong )
원광대학교 의과대학 병리학교실
KMID : 0357920010350020129
Abstract
Background: This experiment was designed to study the cell kinetics of hepatocellular carcinoma (HCC) in both hepatitis B virus X (HBx) transgenic mice and humans.
Methods: The immunohistochemical stain of proliferating cell nuclear antigen (PCNA) and TdT-mediated dUTP-biotin nick end labeling (TUNEL) assay of apoptosis were used on formalin fixed-paraffin embedded tissues.
Results: PCNA labeling indices (PCNA-LI) in the liver of HBx transgenic mice were markedly increased in HCC (11.3%) compare to the dysplastic areas (1.3%) and in the liver of non-transgenic littermates (0.1%). There was no significant difference of PCNA-LI in the dysplastic areas between HCC developed mice and non-HCC developed mice. Apoptosis labeling indices (Apoptosis-LI) in both the dysplastic areas and HCC of HBx transgenic mice were similar to those of non-transgenic littermates. PCNA-LI was markedly increased in human HCC (28.9%) compare to the background of HCC (2.9%) and the control liver (2.9%). Apoptosis-LI was decreased in human HCC (0.3%) compare to the background of HCC (0.4%) and the control liver (1.0%).
Conclusion: There is a marked increase of cell proliferating activity in human HCC and in HCC of HBx transgenic mice, and there is a decrease of apoptosis in human HCC, but not in HCC of HBx transgenic mice.
키워드
Transgenic;Hepatocellular;Carcinoma;Proliferating cell nuclear antigen;Apoptosis
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