9,10-Dimethyl-1,2-Benzanthracene 삽입에 의해 유발된 백서 난소 종양에서 p16과 Rb의 발현양상
Expression of p16 and Rb in 9,10-Dimethyl-1,2-Benzanthracene Induced Rat Ovarian Carcinogenesis
김기권, 김동훈,
소속 상세정보
김기권 ( Kim Ki-Kwon )
동국대학교 의과대학 병리학교실
김동훈 ( Kim Dong-Hun )
동국대학교 의과대학 병리학교실
KMID : 0357920010350020144
Abstract
Background: In order to investigate the roles of p16 and Rb, their expression was evaluated in 9,10-dimethyl-1,2-benzanthracene (DMBA)-induced ovarian cancers of rats.
Methods: DMBA-coated silk was inserted into both ovaries of 20 9-week-old Sprague-Dawley rats. The experimental period lasted 20 weeks. The tumor histology was classified and the expression of p16 and Rb in the ovarian tumors was analyzed by immunohistochemistry and Western blot.
Results: The p16 and Rb labeling index was significantly lower in the ovarian cancers than the normal ovarian surface epithelium of a rat. There were no differences among the cancer types. In Western blot analysis, the expressions of p16 and Rb in ovarian cancers were lower than those in normal ovarian tissue. No correlation was present between p16 and Rb.
Conclusions: The abnormal expression of p16 and Rb occurs in DMBA-induced rat ovarian cancer and might be involved in carcinogenesis.
키워드
Ovary;Neoplasms;9;10-Dimethyl-1;2-benzanthracene;Protein p16;Retinoblastoma protein
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