위의 증식성 병변에서 동소교잡법에 의한 Telomerase mRNA 및 RNA 성분의 검출
In Situ Detection of mRNA and RNA Component of Human Telomerase in Proliferative Lesions of the Stomach
김미숙, 정상우,
소속 상세정보
김미숙 ( Kim Mi-Sook )
전남대학교 의과대학 병리학교실
정상우 ( Juhng Sang-Woo )
전남대학교 의과대학 병리학교실
KMID : 0357920010350040299
Abstract
Background: Prolifertive lesions of the stomach were investigated by in situ hybridization using RNA probes for telomerase components and compared with the results by TRAP（telomeric repeat amplification protocol） assay.
Methods: RNA probes for hTR （human telomerase RAN component） and hTERT （mRNA coding for a catalytic subunit of human telomerase） were made by cloning and in vitro transcription. The probes were applied for in situ hybridization in 23 cases of adenocarcinoma of the intestinal type and adjacent dysplasia, and in the normal and metaplastic mucosa of the stomach.
Results: Telomerase activity by TRAP was positive in all cases of adenocarcinoma, most cases of dysplasia, and many cases of normal mucosa. hTR in situ hybridization showed positive staining in the adenocarcinoma cells, dysplastic cells, a few cells in the proliferation zone of the nomal mucosa, and a few infiltrated lymphocytes. hTERT showed positived staining in the same cells.
Conclusions: Telomerase is expressed in most cases of dysplastic lesions and is thought to be acquired in the early steps of carcinogenesis The expression is noted in a few cells of the normal proliferative zones and the infiftrated lymphocytes, emphasizing the importance of in situ detection of telomerase at the cell level.
키워드
Telomerase;Polymerase chain reaction;mRNA;In situ hybridization
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