Transforming Growth Factor- 1에 대한 항체 및 유전자 치료가 피부 반흔 형성에 미치는 영향
The Effect of Antibody and Gene Therapy for Transforming Growth Factor- 1 on Scar Formation
김준형, 한기환, 안종덕, 이인규, 김은주, 황미열, 박관규,
소속 상세정보
김준형 ( Kim Jun-Hyung )
계명대학교 의과대학 성형외과학교실
한기환 ( Han Ki-Hwan )
계명대학교 의과대학 성형외과학교실
안종덕 ( Ahn Jong-Duck )
계명대학교 의과대학 내과학교실
이인규 ( Lee In-Kyu )
계명대학교 의과대학 내과학교실
김은주 ( Kim Eun-Joo )
계명대학교 의과대학 병리학교실
황미열 ( Hwang Mee-Yul )
계명대학교 의과대학 병리학교실
박관규 ( Park Kwan-Kyu )
계명대학교 의과대학 병리학교실
KMID : 0357920010350050424
Abstract
Background: Transforming growth factor (TGF)- has a large variety of biological functions, including the modulation of inflammation and the immune system, and is presumed to play important roles in repairing wounds and reducing scarring. The objective of this study is to examine the effects of TGF-1 on healing wounds and reducing scarring. We have also analysed the ability of the hemagglutinating virus of Japan (HVJ) liposome mediated antisense oligodeoxynucleotides (ODNs) to specifically inhibit wound-induced expressions of TGF-1 proteins and mRNA in the rat skin.
Methods: Skin wounds were created on the backs of 80 anesthetized rats. The first group of wounds, as the controls, was unmanipulated. The second group of wounds, as positive controls or an excessive scarring model, was injected with TGF-1 subcutaneously. The third group of wounds was injected with anti-TGF-1 antibody subcutaneously. The fourth group of wounds was injected with HVJ liposome mediated antisense ODNs for TGF-1 subcutaneously. The wounds of all groups were bisected and analysed histologically 5, 10, 15, 30, and 50 days after the wounds were made.
Results: All control wounds (TGF-1 or no injection) healed with scarring, whereas the wounds treated with the antibody or antisense ODNs healed with less scar formation compared to the control group. The wounds treated with the antibody or antisense ODNs had fewer macrophages, less collagen and fibronectin contents than the other wounds. Northern blotting and in situ hybridization analysis showed that wound sites treated with HVJ liposome mediated antisense ODNs for TGF-1 exhibited decreased levels of TGF-1 mRNA after injury.
Conclusions: These findings suggest an important new approach to controlling scarring in normal wound healing, complementing the practice of adding exogenous growth factors to chronic wounds in the attempt to inhibit collagen deposition.
키워드
Transforming growth factor beta;Liposomes;Wounds and injuries;Oligoribonucleotides; Antisense
원문 및 링크아웃 정보
등재저널 정보