폐포자충 폐렴에서의 폐포세포자멸사:전자현미경적 Terminal Deoxynucleotidyl Transferase-Mediated dUTP-Biotin Nick End Labeling법의 적용
Apoptosis of Alveolar Cells in Pneumocystis Carinii Pneumonia: Application of Electron Microscopic Terminal Deoxynucleotidyl Transferase-Mediated dUTP-Biotin Nick End Labeling Method
강규헌, 김상표, 권건영,
소속 상세정보
강규헌 ( Kang Kyu-Hun )
계명대학교 의과대학 병리학교실
김상표 ( Kim Sang-Pyo )
계명대학교 의과대학 병리학교실
권건영 ( Kwon Kun-Young )
계명대학교 의과대학 병리학교실
KMID : 0357920010350060496
Abstract
Background: Pneumocystis carinii (P. carinii) attaches to alveolar cells and causes injury to the epithelial cells by direct toxic effects or inhibition of epithelial growth and replication. Although respiratory cell damage or death is a common feature in P. carinii pneumonia, there has been little reports about expression of apoptosis of the lung tissue in the literatures.
Methods: We examined expression of fibronectin and vitronectin in the interaction between P. carinii and alveolar cells, and in situ terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end-labeling (TUNEL) expression of apoptosis in the respiratory cells by immunohistochemistry and pre-embedding immunoelectron microscopy.
Results: Light microscopic (LM) and electron microscopic (EM) immunohistochemical stains for the fibronectin and vitronectin showed strong expressions on the pellicles and tubular extensions of P. carinii and weak expression along the surfaces of type I alveolar cells. LM and EM TUNEL stains showed positive expression in the nuclei of alveolar cells, apoptotic bodies in the cytoplasm of alveolar macrophages and cellular debris in alveolar spaces.
Conclusions: P. carinii induces injury and apoptosis of alveolar cells after attachment of the organisms to host cells, and alveolar macrophages enhance the clearance of apoptotic bodies of alveolar cells as well as phagocytosis and degradation of P. carinii.
키워드
Pneumocystis carinii;In situ nick-end labeling;Apoptosis;Microscopy;immunoelectron
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