위의 저등급 점막 관련 림프조직 림프종에서 Helicobacter pylori 치료에 따른 p16메틸화의 변화
Methylation Analysis of p16/INK4A in Gastric Low-Grade Mucosa-Associated Lymphoid Tissue Lymphomas after Helicobacter pylori Eradication Therapy
김영아, 송인성, 김유선, 박성신, 이보영, 김철우,
소속 상세정보
김영아 ( Kim Young-A )
서울대학교 의과대학 병리학교실
송인성 ( Song In-Sung )
서울대학교 의과대학 내과학교실
김유선 ( Kim You-Sun )
서울대학교 의과대학 내과학교실
박성신 ( Park Sung-Shin )
서울대학교 의과대학 병리학교실
이보영 ( Lee Bo-Young )
서울대학교 의과대학 병리학교실
김철우 ( Kim Chul-Woo )
서울대학교 의과대학 암연구소
KMID : 0357920020360010013
Abstract
Background: Inactivation of p16 has been associated with promoter region hypermethylation in different types of malignancies, including non-Hodgkin´s lymphomas (NHLs). This loss of p16 was found frequently in cases of mucosa-associated lymphoid tissue (MALT) lymphomas. Recent studies indicate that promoter hypermethylation is often an early event in tumor progression, and it has been reported that p16 methylation could be a marker of disease progression in the follow-up of NHLs.
Methods: To investigate the usefulness of p16 methylation in the diagnosis and follow-up of gastric low-grade MALT lymphomas, we analyzed methylation status of p16 using methylation-specific polymeraes chain reaction methods in th sequential biopsy specimens of 13 patients with gastric low-grade MALT lymphomas undergoing Helicobacter pylori eradication therapy.
Results: Five of thirteen cases showed p16 hypermethylation upon diagnosis. In four of five methylation positive cases, abnormal methylation was detected in the specimen even after the treatment, although there were no histologic evidence of disease. This methylation disappeared in the later samples of two of the cases, and they have remained in complete remission. Immunohistochemically, the loss of p16 protein expression was detected in one of three methylation-positive cases, and in none of the methylation-negative cases.
Conclusions: These results suggest that p16 methylation is relatively frequent in low-grade gastric MALT lymphomas, and it may have clinical applications in the management and follow-up of low-grade gastric MALT lymphomas.
키워드
Lymphoma;Mucosa;Associated Lymphoid Tissue-Genes;p16-DNA Methylation
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